摘要
The inhibitory effect and its virulence of chlorine dioxide(ClO_(2))against dry rot of potato were investigated.Potatoes were treated by ClO_(2),then observed for indoor bioassay,scanning electron microscopy(SEM)and transmission electron microscopy(TEM)were used to observe the morphology and ultrastructure of hyphae,and evaluated the control efficiency of ClO_(2) on potato tuber(LK99)dry rot by F.sulphureum pre-treatment.The results showed that the pathogen of potato dry rot was sensitive to ClO_(2),the virulence of regression of y=5.05+7.308x,EC50 and EC90 were 0.3490 and 0.6261 respectively,the treatment of ClO_(2) could significantly inhibit the spore germination and mycelium growth of F.sulphureum,which was in a concentration-dependent manner,SEM and TEM observed that the morphology and ultrastructure of F.sulphureum hyphae were regularly damaged by ClO_(2),in vivo experiment further indicated that ClO_(2) could effectively control the dry rot of potato tubers with F.sulphureum,and ClO_(2) at the concentration of 0.75 ug/mL could significantly reduce the incidence of potato tuber dry rot and lesion expansion rate.The study showed that ClO_(2) could greatly against the pathogen of F.sulphureum,which could provide a scientific theoretical basis for the safe and efficient application of ClO_(2) in the prevention and control of potato diseases after harvest.
基金
supported by the Youth Fund Project of Gansu Academy of Agricultural Sciences(No.2015GAAS23)
Special Fund for China Agriculture Research System(Cars-10)
Special Fund for Agro-scientific Research in the Public Interest(201003077)
the National Natural Science Foundation of China(No.31760476).
