摘要
目的观察重度中暑猪早期胰岛功能的变化。方法将12只健康CV级雄性巴马小型猪按随机数字表法分为对照组(6只)、模型组(6只)。将对照组置于室温下,模型组放入恒温恒湿气候舱中,在温度为44~47℃,相对湿度为(65±5)%的条件下建立猪重度中暑模型,以模型组核心温度>42℃为造模成功,即实验结束。监测两组一般情况和核心温度,实验结束后采集两组血液标本送检,比较其血糖、胰岛素水平变化;采用苏木精-伊红染色观察两组胰岛、肝、肾组织形态,采用免疫组织化学染色观察两组胰岛中胰岛素水平。结果模型组进入恒温恒湿气候舱后,核心体温呈升高趋势,对照组核心温度无变化。模型组核心温度>42℃时出现精神萎靡不振、步态不稳、结膜充血、角膜反射减弱或消失,对照组无异常表现。对照组实验前后血糖及血液中胰岛素水平比较,差异无统计学意义(P>0.05)。实验后,模型组血糖水平高于实验前,血液中胰岛素水平低于实验前,且模型组血糖水平高于对照组,血液中胰岛素水平低于对照组,差异有统计学意义(P<0.05)。实验后,对照组胰岛、肝、肾组织无明显改变;模型组胰岛边缘不清,形态不规则,细胞水肿,少量粒细胞浸润,毛细血管瘀血;肾小管与集合管扩张,血管内可见血栓形成,上皮细胞颗粒变性,少量炎症细胞浸润;肝小叶结构紊乱,肝窦轻度瘀血扩张,肝细胞轻度水肿、颗粒变性,见淋巴细胞浸润。免疫组织化学染色结果显示,对照组观察到数量较多、呈棕黄色的胰岛素阳性表达,细胞排列紧密均匀,形态完整;模型组观察到胰岛着色区域少,形态不规则。模型组胰岛中胰岛素平均光密度值低于对照组,差异有统计学意义(P<0.05)。结论猪在重度中暑后早期即出现胰岛组织损伤、胰岛素水平下降、血糖升高,可能与胰岛功能受损有关。
Objective To observe the changes of early islet function in pigs with severe heat stroke.Methods Twelve healthy CV level male Bama miniature pigs were divided into control group(six pigs)and model group(six pigs)according to random number table method.The control group was placed at room temperature,and the model group was placed in the climate chamber with constant temperature and humidity.The severe heat stroke model of pigs was established under the conditions of 44-47℃and relative humidity of(65±5)%.The core temperature of the model group>42℃was regarded as the success of modeling,that was the experiment ended.General conditions and core temperature of the two groups were monitored.Blood samples were collected from the two groups for examination after the end of the experiment to compare the changes in blood glucose and insulin levels.hematoxylin-exosin staining was used to observe the tissue morphology of the islets,liver and kidney of the two groups,and immunohistochemical staining was used to observe the expression level of insulin in the islets of the two groups.Results After the model group entered the constant temperature and humidity climate cabin,the core temperature of the model group showed an increasing trend,while the core temperature of the control group did not change.At the core temperature>42℃,the model group showed depression of spirit,unstable gait,conjunctival congestion,and weakened or disappeared corneal reflex,while the control group showed no abnormal symptoms.There was no significant difference in blood glucose and insulin levels in the control group before and after the experiment(P>0.05).After the experiment,the blood glucose level of the model group was higher than before,and the blood insulin level of the model group was lower than before,and the blood glucose level of the model group was higher than that of the control group,and the blood insulin level of the control group was lower than that of the control group,the difference was statistically significant(P<0.05).After the experiment,there were no significant changes in islet,liver and kidney tissue in the control group.The islet margin of model group was not clear,the shape was irregular,the cell edema,a few granulocytes infiltration,and the blood stasis of the capillary.The renal tubules and collecting ducts were dilated,thrombosis was observed in the vessels,epithelial granules were degenerative,and a few inflammatory cells were infiltrated.The structure of hepatic lobules was disordered,the hepatic sinuses were slightly stasis and dilated,the hepatic cells were slightly edema and granular degeneration,and lymphocyte infiltration was observed.Immunohistochemical staining results showed that in the control group,a large number of positive expressions of brown insulin was observed.The cells were closely arranged and uniform with complete morphology.In the model group,the islets were less stained and irregular in shape.The average optical density of insulin in islets in model group was lower than that in control group,and the difference was statistically significant(P<0.05).Conclusion Islet tissue damage,decreased insulin expression and elevated blood glucose occurred in early stage after severe heat stroke in pigs,which may be related to the impaired islet function.
作者
乔泽渊
倪军
张静
谢国旗
QIAO Zeyuan;NI Jun;ZHANG Jing;XIE Guoqi(Graduate School,Xinxiang Medical University,He’nan Province,Xinxiang453000,China;Department of Blood Endocrinology,Army 83rd Group Military Hospital,He’nan Province,Xinxiang453000,China)
出处
《中国医药导报》
2021年第23期4-7,32,I0002,共6页
China Medical Herald
基金
陆军后勤部军队后勤科研计划项目(CLJ20J026)。
关键词
重度中暑
胰岛素
血糖
猪
Severe heat stroke
Insulin
Blood glucose
Pig
