摘要
Microbial transformation is a useful tool for structure modification of active natural products.Z-Butylidenephthalide is the main active constituent of Angelica sinensis.In the present work,a pair of new oxidized metabolites,namely(-)(11R)-(Z)-11-hydroxy-butylidenephthalide(1a)and(+)(11S)-(Z)-11-hydroxy-butylidenephthalide(1b),were obtained from microbial transformation for region-selective 11-hydroxylation of Z-butylidenephthalide by Aspergillus niger CGMCC 3.739.Their structures,including absolute configurations,were elucidated by extensive analysis of HR-ESI-MS,NMR spectra,and the modified Mosher’s method.This is the first study on the microbial transformation of Z-butylidenephthalide,and these findings offer a tool for region-selective 11-hydroxylation of Z-butylidenephthalide.
基金
This work was financially supported by grants from the National Key Research and Development Program of China(2018 YFA0903200/2018YFA0903201)
the National Natural Science Foundation of China(81925037/31900284)
Chang Jiang Scholars Program(Young Scholar)from the Ministry of Education of China(Hao Gao,2017)
National High-level Personnel of Special Support Program(2017RA2259)
Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Y036)
K.C.Wong Education Foundation(Hao Gao,2016).
