摘要
目的建立藏药兔耳草环烯醚萜苷类主要成分(梓醇、桃叶珊瑚苷)的含量测定方法,并对不同基原兔耳草及不同药用部位进行对比研究。方法采用Waters Atlantis T3 C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸水(1∶99);检测波长:210 nm;流速:1 mL·min^(-1)。采用单因素方差分析方法进行数据分析。结果所建立的梓醇、桃叶珊瑚苷含量测定方法分离度高,在测定的浓度范围内线性关系好,精密度、重复性、稳定性的RSD均小于2.0%。梓醇、桃叶珊瑚苷平均加样回收率分别为102.94%、100.85%。各基原兔耳草环烯醚萜苷类主要成分(梓醇、桃叶珊瑚苷)比较:全缘兔耳草>紫叶兔耳草>短穗兔耳草>圆穗兔耳草>革叶兔耳草>短筒兔耳草。同一基原不同药用部位比较:梓醇含量地下部位优于地上部位,而桃叶珊瑚苷含量地上部位优于地下部位。结论所建立的方法快速、简便、重复性好,可用于藏药兔耳草梓醇、桃叶珊瑚苷的含量测定,为兔耳草的质量控制提供参考。短筒兔耳草环烯醚萜苷类成分含量低于其他5种基原。建议将各基原兔耳草均作为藏药兔耳草使用。
Objective To establish the method for determination of the contents of iridoid glycosides(catalpol and aucubin)in Tibetan medicine Lagotidis herba,and to compare the main components of iridoid glycosides in Lagotidis herba and the different parts.Methods The Waters Atlantis T3 C_(18)chromatographic column(4.6 mm×50 mm),the acetonitrile-0.1%phosphate water as flow phase,the detection wavelength of 210 nm,and a flow rate of 1 mL·min^(-1),were used to set up an HPLC method for determination of iridoid glycosides in Lagotidis herba.The obtained data were analyzed by one-way ANOVA.Results The established detection method for catalpal and aucubin could separate the two components from other chromatographic peaks,and the linear relationships were good within the ranges of the determined concentration.RSDs of the precisions,repeatability and stabilities were all less than 2.0%.And the average recovery rates of catalpal and aucubin were 102.94%and 100.85%.The method could be used as a quantitative analysis method for the medicinal materials of Lagotidis herba.In the samples with different origin,order of the contents of iridoid glycosides(catalpol and aucubin)were Lagotis integra>Lagotis praecox>Lagotis brachystachya>Lagotis ramalana>Lagotis alutacea>Lagotis brevituba.In different medical parts,the average content of catalpol in underground part was higher than aboveground part.The average content of aucubin in aboveground part was higher than that in underground part.Conclusion The established method can be used to determine the contents of catalpol and aucubin in Lagotidis herba,which can provide reference for the quality control.The contents of iridoid glycosides of Lagotis brevituba is the lowest among the six origins.Therefore,the six origins all can be used.
作者
何芳
齐景梁
李倩
杨吉玉
高必兴
周娟
HE Fang;QI Jingliang;LI Qian;YANG Jiyu;GAO Bixing;ZHOU Juan(Sichuan Institute for Food and Drug Control/Key Laboratory of Quality Evaluation of Chinese Patent Medicines,NMPA,Chengdu 611731 Sichuan,China;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610072 Sichuan,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2021年第7期1019-1023,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家食药监总局药化司专项“特色民族药材检验方法示范性研究”。
关键词
兔耳草
基原
含量测定
梓醇
桃叶珊瑚苷
高效液相色谱法
Lagotidis herba
origins
content determination
catalpol
aucubin
the method of high performance liquid chromatography(HPLC)
