摘要
维生素C是一种人体必需的维生素,在食品制药等领域拥有巨大的市场。工业上维生素C主要以微生物发酵生产的2-酮基-L-古龙酸为前体,然后通过内酯化反应获得。微生物发酵中,山梨糖途径和葡萄糖酸途径因为转化率高一直是研究的热点。文中从维生素C生物合成相关脱氢酶的角度阐述了:山梨糖途径和葡萄糖酸途径中关键脱氢酶在定位、底物谱、辅因子和电子传递上的特点;山梨糖途径和葡萄糖酸途径中面临的主要问题和改造策略等。最后讨论了维生素C生物合成中山梨糖途径和葡萄糖酸途径可能的研究方向。
Vitamin C is an essential vitamin for human beings.It has a huge market in the fields of food and pharmaceuticals.2-keto-L-gulonic acid is an important precursor to produce vitamin C by microbial fermentation in industrial.In microbial fermentations,the L-sorbose pathway and the D-gluconate pathway have been the focus of research because of high yield.This article aims at stating recent research progress in dehydrogenases related to biosynthesis of vitamin C in the L-sorbose pathway and the D-gluconate pathway.The properties of dehydrogenase in terms of localization,substrate specificity,cofactors,and electron transport carrier are elaborated.And then,the main problems and strategies are reviewed in the L-sorbose pathway and in the D-gluconate pathway.Finally,future research on the dehydrogenases in the biosynthesis of vitamin C through L-sorbose pathway and D-gluconate pathway is discussed.
作者
陈玥
周景文
陈坚
Yue Chen;Jingwen Zhou;Jian Chen(National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;Science Center for Future Foods,Jiangnan University,Wuxi 214122,Jiangsu,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2021年第6期1827-1844,共18页
Chinese Journal of Biotechnology
基金
国家自然科学基金(No.31830068)
国家优秀青年科学基金(No.21822806)资助。
