摘要
目的:对枇杷叶多糖的提取工艺进行优化,并研究其抗氧化活性。方法:采用超声波辅助醇提取法提取枇杷叶中的多糖,在单因素试验的基础上,以液料比、超声时间、超声功率、乙醇浓度为影响因素,以Box-Behnken设计中心组合建立数学模型,以枇杷叶多糖的提取率为响应值,优选枇杷叶多糖的提取条件;并通过测定DPPH自由基和羟自由基的清除效果评价枇杷叶多糖体外抗氧化活性。结果:枇杷叶多糖超声提取最佳工艺为液料比25∶1mL/g、超声功率为425W、超声时间为13min、乙醇浓度为50%,枇杷叶多糖的提取率为3.36%;枇杷叶多糖对DPPH自由基和羟基自由基具有较好的清除能力。结论:该优化工艺稳定、可行,可用于枇杷叶多糖的高效提取。
Objective:The extraction process of loquat leaf polysaccharide was optimized and its antioxidant activity was studied.Methods:The polysaccharides from loquat leaves were extracted by ultrasonic method.On the basis of single factor experiment,the ratio of liquid to solid,ultrasonic time,ultrasonic power and ethanol concentration were taken as the influencing factors,and the mathematical model was established by Box-Behnken design center combination.The extraction rate of polysaccharides from loquat leaves was taken as the response value to optimize the extraction conditions.The antioxidant activity of Eriobotrya japonicum polysaccharides in vitro was evaluated by determining the scavenging effect of DPPH free radical and hydroxyl free radical.Results:The optimal extraction conditions were as follows:liquid/solid ratio was 25∶1mL/g,ultrasonic power was 425W,ultrasonic time was 13min,and ethanol concentration was 50%.The extraction rate of polysaccharides from Eriobotrya japonica leaves was 3.36%.Polysaccharides from Eriobotrya japonica leaves had better scavenging ability on DPPH and hydroxyl radicals.Conclusion:The optimized process is stable and feasible,which can be used for efficient extraction of loquat leaf polysaccharide.
作者
王娟
袁绍莉
王丹
WANG Juan;YUAN Shaoli;WANG Dan(Pharmaceutical and Food College,Xuzhou Vocational College of Bioengineering,Xuzhou 221006)
出处
《中国食品添加剂》
CAS
北大核心
2021年第6期47-54,共8页
China Food Additives
