摘要
本文探讨了夏枯草粗多糖(PVCP)对镉诱导肾小管上皮细胞RPTEC/TERT1炎症反应的调节作用。CCK-8法测定氯化镉与PVCP对RPTEC/TERT1作用24 h后的细胞存活率。将细胞设置为对照组(空白培养基)、模型组(8μmol/L氯化镉)及PVCP高、中、低剂量干预组(200、100、50μg/mL PVCP加8μmol/L氯化镉)。测定细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)酶活力的变化,细胞上清液中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)、肿瘤坏死因子α(TNF-α)的水平以及细胞内iκB及p65表达水平。结果显示,夏枯草样品中多糖含量为2.14%。氯化镉剂量依赖性抑制RPTEC/TERT1细胞活力。与对照组相比,镉处理组细胞内SOD、CAT含量显著升高(p<0.01),IL-6、IL-1β、IL-18及TNF-α表达水平分别升高了9.53倍、8.80倍、10.86倍和1.17倍(p<0.01)。同时,NF-κB信号通路关键蛋白iκB及p65的表达显著升高(p<0.05)。与模型组相比,PVCP干预组SOD及CAT活力下降(p<0.01),IL-1β、IL-6、TNF-α、IL-18分泌下降(p<0.01),iκB及p65的表达降低(p<0.05)。结果表明,PVCP可改善镉诱导的RPTEC/TERT1细胞炎症反应,该效应可能与其抗氧化能力及对NF-κB信号通路的抑制有关。
The regulatory effects of crude polysaccharides extracted from Prunella vulgaris L.(PVCP)on cadmium-induced inflammatory responses in human renal proximal tubule epithelial cells RPTEC/TERT1 were investigated.Cell counting kit-8(CCK-8)assay was used to detect the survival rate of RPTEC/TERT1 cells after cadmium or PVCP treatment for 24h.Control group(blank culture medium),model group(8μmol/L cadmiumchloride)and high-,medium-and low-dose groups of PVCP(8μmol/L cadmiumchloride+200,100,50μg/mL PVCP)were set in this study.The catalase(CAT)and superoxide dismutase(SOD)activities of RPTEC/TERT1 cells,interleukin(IL)-6,IL-1β,IL-18 and tumor necrosis factor(TNF)-αlevels in the supernatant and the protein expression levels of iκB and p65 were evaluated.The results showed that the content of polysaccharides in Prunella vulgaris L.sample was 2.14%.The viability of RPTEC/TERT1 cells was significantly inhibited by cadmium chloride in a dose-dependent manner.In addition,the activities of SOD and CAT of RPTEC/TERT1 cells in the cadmium treated group were significantly decreased(p<0.01)in comparison with control group,while the expression levels of IL-6,IL-1β,IL-18 and TNF-α wereincreased by 9.53 times,8.80 times,10.86 times and 1.17 times,respectively(p<0.01).Meanwhile,the expression levels of key proteins iκB and p65 in NF-κB signaling pathway were significantly increased(p<0.05).Compared to the model group,the activities of SOD and CAT were significantly increased(p<0.01),while IL-1β,IL-6,TNF-α and IL-18 as well as iκB and p65 were significantly decreased(p<0.05)after PVCP treatment.In conclusion,PVCP could ameliorate cadmium-induced inflammation in RPTEC/TERT1 cells,and its anti-inflammatory effect may be related to antioxidant response and inhibition of NF-κB signaling pathway.
作者
池慧钦
黎姿茵
宋佳
赖月妃
王延
杨佳妮
万宇
何志妮
卫秦芝
吴炜亮
杨杏芬
CHI Hui-qin;LI Zi-yin;SONG Jia;LAI Yue-fei;WANG Yan;YANG Jia-ni;WAN Yu;HE Zhi-ni;WEI Qin-zhi;WU Wei-liang;YANG Xing-fen(Food Safety and Health Research Center,Key Laboratory of Cosmetics Safety Evaluation of National Medical Products Administration,Guangdong Provincial Key Laboratory of Tropical Disease Research,School of Public Health,Southern Medical University,Guangzhou 510515,China;Department of Public Health and Preventive Medicine,School of medicine,Jinan University,Guangzhou 510632,China)
出处
《现代食品科技》
CAS
北大核心
2021年第6期1-9,共9页
Modern Food Science and Technology
基金
国家重点研发计划项目(2018YFC1603101)
广州市科技计划项目(201803010107)
广东省重点领域研发计划项目(2019B020210002)
国家自然科学基金资助项目(81872642)。
关键词
夏枯草粗多糖
镉
肾小管上皮细胞
炎症反应
NF-κB信号通路
Prunella vulgaris L.crude polysaccharides
cadmium
renal tubular epithelial cells
inflammation
NF-κB signaling pathway
