摘要
目的探讨5-羟色胺(5-HT)在小鼠慢性背根神经节压迫(CCD)模型中的作用机制。方法将L型不锈钢棍插入小鼠右侧L3和L4椎间孔,持续压迫背根神经节(DRG)建立小鼠CCD模型。在CCD手术前1 d及手术后1、3、5、7 d向小鼠胫骨前区皮内注射10μL 5-HT(低剂量浓度为0.003 g/L,高剂量浓度为0.3 g/L)后,录像分析小鼠对注射5-HT后所产生的痒与痛行为学;免疫荧光实验检测小鼠DRG中H4蛋白的表达;实时PCR检测CCD模型小鼠压迫5 d后双侧L3和L4 DRG内5-HTr1a和5-HTr2a的表达。结果与手术前1 d比较,低浓度5-HT在CCD手术后3 d可以明显增加痒行为;高浓度5-HT在CCD手术后1 d可以明显增加痒行为(P<0.05)。与手术前1 d比较,低浓度5-HT在小鼠CCD手术后1 d和3 d可以明显增加痛行为(P<0.05);高浓度5-HT在小鼠CCD手术后1 d可以明显增加痛行为(P<0.05)。与对侧DRG比较,压迫5 d的DRG中H4蛋白,5-HTr1a、5-HTr2a mRNA表达均显著升高(均P<0.05)。结论受压迫DRG神经元H4、5-HTr1a和5-HTr2a表达上调,引起感受野对5-HT诱发痛与痒的敏化。
Objective To investigate the mechanism of 5-hydroxytryptamine(5-HT)in the chronic compression of dorsal root ganglion(CCD)in mice.Methods An L-shaped stainless steel rod was inserted into the L3 and L4 intervertebral foramen on the right side of each CCD model mouse,establishing continuous compression of the dorsal root ganglion(DRG).For this behavioral experiment,model mice were divided into two groups.All mice received an initial intradermal injection of 10μL 5-HT.Subsequently,the low dose group received 0.003 g/L,and the high-dose group received 0.3 g/L injections into the anterior tibial region 1 day before the CCD operation,and 1,3,5,and 7 days after the operation.Itching and pain response behaviors after 5-HT injection were analyzed by video recording,and H4 protein expression in the DRG region was detected by immunofluorescence.RT-PCR was used to detect 5-HTr1a and 5-HTr2a expression in bilateral L3 and L4 DRG of CCD model mice over 5 days.Results Low dose 5-HT significantly increased itching behavior 3 days after CCD relative to 1 day before,and high dose 5-HT significantly increased itching behavior by 1 day after CCD.Low dose 5-HT significantly increased pain response behavior at 1 and 3 days post-CCD relative to 1 day before CCD,and high dose 5-HT significantly increased pain response behavior at 1 day after CCD.Compared with expression in contralateral DRG,H4 protein expression and 5-HTr1a and 5-HTr2a mRNA expression were significantly increased after 5 days of compression in compressed DRG.Conclusion Expression of H4 protein,and 5-HTr1a and 5-HTr2a mRNA is upregulated in compressed DRG neurons,which sensitizes the receptive field to 5-HT induced pain and itching.
作者
王涛
陶金
方烨红
崔欢
马超
WANG Tao;TAO Jin;FANG Yehong;CUI Huan;MA Chao(Department of Human Anatomy,Histology and Embryology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine,Peking Union Medical College,Beijing 100005,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2021年第6期507-510,共4页
Journal of China Medical University
基金
国家自然科学基金(81771205)
中国医学科学院医学与健康科技创新工程协同创新团队项目(CIFMS#2017-I2M-4-005(TW))。
关键词
5-羟色胺
小鼠
慢性背根神经节压迫
疼痛
痒
5-hydroxytryptamine
mice
chronic dorsal root ganglion compression
pain
itching
