摘要
从青岛市黄岛区积米崖码头采集的海水样本中筛选出具有高效石油降解能力的菌种,命名为C2,通过单因素实验,研究不同盐度、温度、pH值、柴油投加量、菌液投加量对菌种生物量的影响。在此基础上进行因子分析与响应曲面优化,得出当pH值为6.89、菌液投加量体积分数为1.55%、温度为30.4℃时,C2的OD_(600)达到最大值0.978。在最佳条件下,C2游离菌种5 d的石油降解率为32.94%。为进一步提高降解率,用桂圆壳作为载体固定游离菌种,固定化石油降解菌5d的降解率为50.51%,大于游离菌种的5d降解率,固定化石油降解菌10 d的降解率高达64.65%。经降解动力学研究,固定化石油降解菌降解柴油的反应速率符合二级反应。
A strain named C2 was selected from seawater samples collected from Jimiya Wharf in Huangdao District,Qingdao City,which has high oil degradability.The effects of different salinity,temperature,pH,diesel oil dosage and bacterial liquid dosage on the strain biomass were studied by single factor experiments.On this basis,factor analysis and response surface optimization were carried out,and it was concluded that when the pH was 6.89,the bacterial liquid dosage was 1.55%,and the temperature was 30.4℃,the OD _(600) of C2 reached the maximum value of 0.978.Under the optimum conditions,the oil degradation rate of C2 free bacterial species in 5 d was 32.94%.In order to further improve the degradation rate,using cinnamon shell as a carrier to immobilize free bacteria,the degradation rate of immobilized oil-degrading bacteria in 5d was 50.51%,which was greater than that of free bacteria in 5 d,and the degradation rate of immobilized oil-degrading bacteria in 10d was as high as 64.65%.The degradation kinetics study showed that the rate of degradation of diesel oil by immobilized petroleum-degrading bacteria conformed to the second-order reaction.
作者
陈曦
陈云倩
刘其庭
吕玲
Chen Xi;Chen Yunqian;Liu Qiting;Lyu ling(School of Safety and Environmental Engineering,Shandong University of Science and Technology,Qingdao 266590,China;Qingdao West Coast New Area Natural Resources Bureau,Qingdao 266500,China)
出处
《山东化工》
CAS
2021年第10期260-264,共5页
Shandong Chemical Industry
关键词
石油污染
石油降解菌
响应曲面
固定化
降解动力学
petroleum pollution
petroleum degrading bacteria
response surface
immobilization
degradation kinetics
