摘要
泛素/26S蛋白酶体途径(ubiquitin/26S proteasome pathway,UPP)调控植物生长发育以及抗病抗逆反应等过程,多聚泛素蛋白(UBQ)为泛素单体聚合而成的聚泛素蛋白,主要参与UPP途径的蛋白质水解过程,是一个可重复利用的信号分子,可以识别靶蛋白。为鉴定橡胶草中UBQ基因的结构与功能,本研究从橡胶草品系‘1151’中克隆得到一个多聚泛素基因,命名为‘TkUBQ6’。该基因编码区cDNA为462 bp,编码153个氨基酸。序列比对分析发现,不同物种的UBQ蛋白具有高度的相似性,而TkUBQ6的蛋白序列与同属菊科的莴苣Ls UBQ6相似性达到100%。采用实时荧光定量RT-PCR(RT-qPCR)对TkUBQ6基因的表达模式进行分析发现,该基因在橡胶草不同组织中均有表达。在PEG-6000模拟干旱胁迫、甘露醇介导的渗透压胁迫以及NaCl高盐胁迫处理下,TkUBQ6表达显著提高。在植物激素茉莉酸甲酯(MeJA)和脱落酸(ABA)诱导下,TkU-BQ6基因表达也明显上调;而乙烯利(ET)处理后,该基因的表达在24 h内先显著下调之后小幅度上调。本研究结果表明,TkUBQ6参与橡胶草的抗逆和激素信号的转导过程,为进一步解析橡胶草TkUBQ6的生物学功能提供了参考依据。
The ubiquitin/26 S proteasome pathway(UPP)regulates plant growth and development and disease resistance and stress tolerance.Polyubiquitin protein(UBQ)is a polyubiquitin protein synthesized by the polymerization of ubiquitin monomer,mainly involved in proteolysis in UPP.It is a reusable signal molecule that recognizes a target protein.In order to identify the structure and function of UBQ in Taraxacum kok-saghyz Rodin(TKS),a polyubitin gene TkUBQ6 was cloned from TKS line’1151’in this study,which ORF is 462 bp,encoding 153 amino acids.Sequence comparison indicated that UBQ protein from different species had a high degree of similarity,while TkUBQ6 shared 100%similarity with the Compositae family lettuce LsUBQ6.RT-qPCR analysis showed that TkUBQ6 was expressed in different tissues of TKS.Under PEG-6000 simulated drought stress,mannitol-medi ated osmotic stress and NaCl high-salt stress treatments,the expression of TkUBQ6 was significantly increased.Moreover,TkUBQ6 expression was remarkably upregulated by plant hormones methyl jasmonate(MeJA)and abscisic acid(ABA).However,its expression was significantly down-regulated within 24 h after ethephon(ET)treatment,and then it was slightly upregulated.These results indicate that TkUBQ6 is involved in the process of stress response and hormone signal transduction in TKS.This study provides a reference for further analysis of the biological function of TkUBQ6 in TKS.
作者
王肖肖
覃碧
杨玉双
张继川
聂秋海
刘实忠
Wang Xiaoxiao;Qin Bi;Yang Yushuang;Zhang Jichuan;Nie Qiuhai;Liu Shizhong(Rubber Research Institute,Chinese Academy of Tropical Agricultural Science,Haikou,571101;College of Tropical Crop,Hainan University,Haikou,570228;College of Materials and Engineering,Beijing University of Chemical and Technology,Beijing,100029;Beijing Linglong Dandelion Technology and DevelopmentCo.,Ltd.,Beijing,101102)
出处
《分子植物育种》
CAS
北大核心
2021年第10期3266-3273,共8页
Molecular Plant Breeding
基金
国家重点研发专项(2017YFB0306901)
中国热带农业科学院橡胶研究所基本科研业务费专项(1630022019015,1630022018005)
北京玲珑蒲公英科技发展有限公司研发课题(橡胶草种质收集,创新与良种繁育)共同资助。
