摘要
目的探讨早期胃癌患者血清miR-633和miR-601的表达及其临床价值。方法选取儋州市人民医院收治的胃癌患者116例作为胃癌组,根据胃癌病情进展分为早期胃癌组(n=48)和进展期胃癌组(n=68),另选择50例健康体检者作为对照组。采用实时定量PCR法检测各组血清miR-633及miR-601表达水平,化学发光法测定糖类抗原72-4(CA72-4)及糖链抗原19-9(CA19-9)水平,分析其对早期胃癌的诊断价值。Pearson相关分析早期胃癌患者血清miR-633、miR-601水平与CA72-4及CA19-9的相关性。结果胃癌组血清miR-633表达水平(1.93±0.86 vs.0.62±0.14)、miR-601表达水平(9.16±2.50 vs.3.80±1.27)、血清CA72-4水平(30.74±7.35 vs.3.28±0.85,U/mL)及CA19-9水平(51.38±12.95 v s.15.60±5.83,U/mL)均明显高于对照组(均P<0.001)。进展期胃癌组血清miR-633表达水平(2.58±1.16 v s.1.24±0.57)、miR-601水平(10.92±3.48 vs.7.15±1.96)、血清CA72-4水平(40.52±10.35 vs.16.65±4.70,U/mL)及CA19-9水平(64.15±17.42 vs.36.20±10.18,U/mL)均明显高于早期胃癌组(均P<0.001)。ROC曲线显示,血清miR-633、miR-601、CA72-4及CA19-9四项联合诊断早期胃癌的曲线下面积(0.917,95%CI:0.857~0.975)最大,其敏感度和特异度分别为95.7%和82.6%。相关分析显示,胃癌患者血清miR-633、miR-601水平与CA72-4及CA19-9均呈正相关(r=0.760、0.712、0.828、0.725,P<0.001)。结论早期胃癌患者血清miR-633、miR-601、CA72-4及CA19-9水平明显升高,四项联合检测对早期胃癌诊断具有较高的临床价值。
Objective To investigate the expression and clinical value of serum microRNA(miR)-633 and miR-601 in patients with early gastric cancer.Methods We enrolled 116 patients with gastric cancer admitted to Danzhou people's hospital,which divided into early gastric cancer group(n=48)and advanced gastric cancer group(n=68)according to the progress of gastric cancer.We also enrolled 50 healthy people as control group.The levels of serum miR-633 and miR-601 detected by real-time quantitative PCR and carbohydrate antigen 72-4(CA72-4)and carbohydrate antigen 19-9(CA19-9)detected by chemiluminescence were analyzed.R e s ults The levels of serum miR-633(1.93±0.86 vs.0.62±0.14),miR-601(9.16±2.50 vs.3.80±1.27),CA72-4(30.74±7.35 vs.3.28±0.85,U/mL)and CA19-9(51.38±12.95 vs.15.60±5.83,U/mL)in gastric cancer group were significantly higher than control group(all were P<0.001).The levels of miR-633(2.58±1.16 vs.1.24±0.57),miR-601(10.92±3.48 vs.7.15±1.96),CA72-4(40.52±10.35 vs.16.65±4.70,U/mL)and CA19-9(64.15±17.42 vs.36.20±10.18,U/mL)in advanced gastric cancer group were significantly higher than early gastric cancer group(all were P<0.001).The ROC curve showed that the area under curve(0.917,95%CI:0.857~0.975)in four indicators combined diagnosis was the largest,with the sensitivity and specificity of 95.7%and 82.6%respectively.Correlation analysis showed that the levels of serum miR-633 and miR-601 were positively correlated with CA72-4 and CA19-9 in early gastric cancer(r=0.760,0.712,0.828,0.725,P<0.001).Conclusion The levels of serum miR-633,miR-601,CA72-4 and CA19-9 are significantly increased in patients with early gastric cancer,and the combined detection of four indexes showed higher diagnostic value in early gastric cancer.
作者
黄婷婷
彭鑫
符鑫
廖雪霞
羊明娟
HUANG Ting-ting;PENG Xin;FU Xin(Danzhou City People's Hospital,Danzhou,Hainan 571799)
出处
《临床输血与检验》
CAS
2021年第3期359-363,共5页
Journal of Clinical Transfusion and Laboratory Medicine
