摘要
为探索生物防治番茄产后灰霉病,采用柯赫氏法则从罹病的番茄果实上分离得到菌株FQ-2,基于形态学和rDNA-ITS序列,对该菌株进行病原鉴定,采用小麦内生菌解淀粉芽孢杆菌B9601-Y2(Y2)和球花石斛内生菌贝莱斯芽孢杆菌DJB5(DJB5)对番茄灰霉病进行生物防治。FQ-2被鉴定为灰葡萄孢菌(Botrytis cinerea),且Y2和DJB5对番茄产后果实灰霉病均有较好的预防和治疗效果,Y21×10^(7) cfu/mL和DJB51×10^(8) cfu/mL悬浮剂在处理当天接种FQ-2,10天后的防效分别达88.10%和73.47%。在接种灰霉病菌当天再接种1×10^(8) cfu/mL的Y2和1×10^(7) cfu/mL的DJB5,10天后灰霉病的防治效果分别达68.56%和78.74%。研究结果对番茄产后灰霉病的生物防治具有参考作用。
In order to explore the biological control of gray mold caused by Botrytis cinerea on postharvest tomato,strain FQ-2 was isolated from tomato fruit by the Koch’s postulate,and the pathogen was identified based on its morphology and rDNA-ITS sequence.Bacillus amyloliquefaciens B9601-Y2(Y2),an endophyte of wheat and B.velezensis DJB5(DJB5),an endophyte of Dendrobium thyrsiflorum were used for biological control of the disease.By analyses,FQ-2 was identified as Botrytis cinerea,Y2 and DJB5 had good preventive and therapeutic effects on gray mold of tomato.Y2 with 1×10^(7) cfu/mL and DJB5 with 1×10^(8) cfu/mL had 88.10%and 73.47%of prevention effects,respectively,after 10 days when they were inoculated following FQ-2 in the same day.In the curing experiment,on the day of inoculation of B.cinerea,Y2 with 1×10^(8) cfu/mL and DJB5 with 1×10^(7) cfu/mL had 68.56%and 78.74%of curing effect,respectively,after 10 days when they were inoculated following FQ-2 in the same day.These results are of great significance to the postharvest biological control of tomato gray mold.
作者
沈艳
何鹏搏
何鹏飞
吴毅歆
孔宝华
李兴玉
Shahzad Munir
何月秋
Shen Yan;He Pengbo;He Pengfei;Wu Yixin;Kong Baohua;Li Xingyu;Shahzad Munir;He Yueqiu(State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan Agricultural University,Kunming 650201)
出处
《中国农学通报》
2021年第13期102-107,共6页
Chinese Agricultural Science Bulletin
基金
科技部国际合作计划项目“防治植物病害和促进植物生长的微生物开发应用及机制研究”(2009DFA32360)。
关键词
番茄
生物防治
灰霉病
灰葡萄孢菌
解淀粉芽孢杆菌
贝莱斯芽孢杆菌
病原鉴定
tomato
biological control
gray mold
Botrytis cinerea
Bacillus amyloliquefaciens
Bacillus velezensis
pathogen identification
