摘要
目的建立一种较为简单的仅造成毛细胞丧失同时具有内淋巴灌注通路的豚鼠耳聋模型。方法利用耳蜗侧壁打孔经中阶进行内淋巴灌注注射用水或腺病毒液来造成正常豚鼠耳蜗毛细胞损害,引起耳聋。通过基底膜铺片phalloidin染色和Dapi核染色来观察基底膜受损的情况。利用脑干诱发电位进行致聋效果的判定。结果内淋巴灌注可以造成早期单纯的毛细胞丧失,而基底膜结构和支持细胞没有发现形态上的改变。并且毛细胞在基底膜上缺失的分布与灌注的方向相关,在灌注方向上损失严重,而逆向的毛细胞几乎没有损伤,同时损害呈现远离灌注部位,程度减轻的趋势。脑干诱发电位click测试阈值变化也与灌注方向一致。结论这种耳聋模型的方法造成了单纯的毛细胞特别是外毛细胞损害,有利于药物的同期灌注,并且较以往的耳聋模型对耳蜗的损害轻。但是造成毛细胞损害的机理尚不明确,可能和膜迷路的压力增加有关。
Objective to build a simple deafness model with only hair cells loss,at the same time to build infusion pathway into the endolymphatic system of the guinea pig’s cochleae.Methods adenovirus was infused into the endolymphatic system through a hole made on the lateral wall of the guinea pigs’cochlear.Auditory brainstem response(ABR)was used to examine the hearing level of the deafness model.Phalloidin staining of the basal membrane whole mount was processed to observe the hair cell conditions.Dapi staining was used to show cell nuclei on the basal membrane.Result Endolymphatic infusing can cause the loss of hair cells without effecting the construction and morphology of supply cells on the basal membrane in guinea pigs’cochlear.The distribution of losing of hair cells was parallel to the direction of the infusion of drug into the scala media.The subject threshold tested by ABR using click may be changed with the direction of the infusion.Conclusions this deafness model is built with only hair cells loss,especially out hair cells and will benefit for drug infusing at the same time.The damage of construction in cochlear is milder than other models.But the mechanism of hair cells loss in this model is still unknown,it seems that the immediate increasing pressure in endolymphatic system plays a role which maybe an reproduction of the Meniere disease.
作者
庄文杰
丛宁
韩朝
ZHUANG Wenjie;CONG Ning;HAN Zhao(Department of Otolaryngology,Hua Dong Hospital,FU Dan University,Shanghai,200040,China)
出处
《中国中西医结合耳鼻咽喉科杂志》
2021年第2期81-84,95,共5页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
关键词
毛细胞
耳聋模型
豚鼠
灌注
中阶
hair cell
deafness model
guinea pig
infusion
scala media
