摘要
目的探讨左金丸对溃疡性结肠炎(UC)小鼠滤泡辅助性T细胞(Tfh)的调控作用。方法将BALB/c小鼠随机均分为正常组、模型组、左金丸组(3 g·kg^(-1))及美沙拉嗪组(300 mg·kg^(-1)),每组10只。除正常组外其余各组小鼠采用自由饮水的方式给予3%葡聚糖硫酸钠(DSS)溶液,连续7 d,构建UC小鼠模型。7 d后按照相应剂量灌胃给药,每日1次,连续给药7 d。每天称小鼠体质量并观察其一般症状;采用流式细胞术检测Tfh及滤泡调节性T细胞(Tfr)数量;测定小鼠结肠质量、结肠长度,计算结肠质量指数;采用HE染色法进行结肠组织病理学观察;Western Blot法检测小鼠结肠组织Bax、Bcl-2、Caspase-3蛋白表达水平。结果与模型组比较,左金丸组小鼠的结肠质量指数明显降低(P<0.01),结肠长度明显恢复(P<0.01),结肠组织病理评分明显降低(P<0.05);CD4^(+)CD44^(+)ICOS^(+)、CD4^(+)CD44^(+)IL-17^(+)及CD4^(+)CD44^(+)CXCR5^(+)细胞水平明显下降(P<0.05,P<0.01),CD4^(+)CXCR5^(+)Foxp3^(+)及CD4^(+)CXCR5^(+)Blimp-1^(+)细胞水平均明显升高(P<0.05);结肠组织中的Caspase-3、Bax蛋白表达明显下调(P<0.05,P<0.01),Bcl-2/Bax蛋白比值明显升高(P<0.01)。结论左金丸可能通过调控Tfh/Tfr细胞平衡,以及结肠组织的Bax、Bcl-2、Caspase-3蛋白表达水平,发挥对UC小鼠的治疗作用。
Objective To investigate the regulatory effect of Zuojin pill on follicular helper T cells(Tfh)in mice with ulcerative colitis(UC).MethodsBALB/c mice were randomly divided into normal group,model group,Zuojin pill group(3 g·kg^(-1))and mesalazine group(300 mg·kg^(-1)).In addition to the normal group,the other groups of mice drank 3%DSS freely to construct UC model.After 7 days,the drugs were given by gavage according to the corresponding doses for 7 days.Every day,the body mass of mice was weighed and the clinical symptoms of mice were observed.The number of Tfh and Tfr cells were detected by flow cytometry.After that,the mice were killed and the colon tissue was sampled,the colon weight,colon length and intestine weight index were measured and calculated.The histopathology of colon was observed by HE staining.The protein levels of Bax,Bcl-2 and Caspase-3 were detected by Western Blot.ResultsCompared with the model group,the mice in Zuojin pill group have reduced the index of colon weight significantly(P<0.01),greatly recovered colon length(P<0.01),and evidently descended injury scores(P<0.05);CD4^(+)CD44^(+)ICOS^(+),CD4^(+)CD44^(+)IL-17^(+)and CD4^(+)CD44^(+)CXCR5^(+)cells decreased(P<0.05,P<0.01),CD4^(+)CXCR5^(+)Foxp3^(+),CD4^(+)CXCR5^(+)Blimp-1^(+)cells increased(P<0.05);Bax,Caspase-3 protein levels decreased(P<0.05,P<0.01),Bcl-2/Bax protein ratio increased(P<0.01).Conclusion Zuojinpill may play a role in the treatment of UC by regulating the balance of Tfh/Tfr cells and the protein levels of Bax,Bcl-2 and Caspase-3 in colon tissue.
作者
刘素萍
王海燕
赵海梅
钟友宝
龙健
左铮云
刘端勇
LIU Suping;WANG Haiyan;ZHAO Haimei;ZHONG Youbao;LONG Jian;ZUO Zhengyun;LIU Duanyong(Department of Postgraduate,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;School of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;Laboratory Animal Science and Technology Center,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;College of Science and Technology,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2021年第3期301-306,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(81803988,81760838,81460679)
江西省卫生计生委中医药科研课题(2015A246)
江西省自然科学基金项目(20181BAB205082,20171BAB205088)
江西省教育厅科技项目(GJJ151564)。
