摘要
目的探讨低浓度乙醇对糖尿病大鼠肺纤维化的影响。方法应用24只SD大鼠复制Ⅰ型糖尿病大鼠模型,平均分为正常对照组(CON组)、低剂量乙醇组(EtOH组)、糖尿病组(DM组)和糖尿病+低剂量乙醇组(DM+EtOH组)四组,每组6只。分别喂养6~12周。称取大鼠肺湿重及体重,计算肺系数;HE染色观察肺脏形态变化,Western blot检测肺脏组织线粒体乙醛脱氢酶2(ALDH2)蛋白表达。天狼猩红染色观察肺脏纤维化变化。ELISA法测定肺脏组织AGE含量变化。结果与CON组相比,DM组大鼠肺系数明显增大,肺组织结构破坏明显,肺组织ALDH2蛋白表达降低,12周组较6周组损伤加重。12周DM组肺脏AGE含量和胶原纤维增加。与DM组相比,DM+EtOH组肺组织病理改变减轻,ALDH2蛋白表达升高,胶原纤维和AGE含量减少。结论低浓度乙醇可减轻糖尿病大鼠肺组织形态结构变化,肺纤维化程度减轻,可能与其抑制线粒体ALDH2表达有关。
Objective To investigate the effect of low concentration of ethanol on lung fibrosis in diabetic rat model.Methods Type Ⅰ diabetic rats were prepared in 24 rats and grouped into 4 groups:normal control group(CON group),low dose ethanol group(EtOH group),diabetes mellitusgroup(DM group),diabetes plus low dose ethanol group(DM+EtOH group),6 rats in each group.The rats were feed for 6 weeks and 12 weeks respectively.The lung wet weight and body weight of rats were weighed,and the lung coefficient was calculated.The changes of lung morphology were observed by HE staining method,ALDH2 protein expression was measured by Western blot.The changes of lung fibrosis were measured by Sirius red staining method.The lung AGE content was measured by ELISA method.Results Compared with the CON group,the lung coefficient in the DM group was increased,obvious damage of lung tissue structure was observed,ALDH2 protein expression was decreased,when compared with the rats in DM-6 weeks group,the injury was aggravated in DM-12 weeks group.In DM-12 weeks group,the collagen fiber numbers and AGE contents were increased.Compared with the DM group,the lung pathological change was alleviatedin DM+EtOH group,ALDH2 protein expression was increased,the collagen fiber numbers and AGE content were reduced.Conclusions Low dose ethanol could attenuate morphological and structural changes of lung tissue and lung fibrosis degree in diabetic rats,which may be related to depress ALDH2 expression in mitochondria.
作者
宫蓓蕾
王文连
刘黎明
赵成岭
胡俊锋
GONG Bei-lei(Department of respiratory and critical care medicine,the first affiliated hospital of Bengbu,Medical College,Bengbu,,Anhui,233030,China)
出处
《齐齐哈尔医学院学报》
2020年第24期3037-3040,共4页
Journal of Qiqihar Medical University
基金
安徽省自然科学基金项目(1808085MH280)
蚌埠医学院第一附属医院科技发展基金项目(Byyfykj201702)。
关键词
糖尿病
乙醛脱氢酶2
大鼠
肺纤维化
Diabetes mellitus
Acetaldehyde dehydrogenase 2
Rat
Lung fibrosis
