摘要
目的研究透明细胞肾细胞癌(ccRCC)中微小RNA(miR)-4313及T-box转录因子15(TBX15)的表达及临床意义。方法应用荧光定量PCR法检测2018年3月—2019年12月辽宁省本溪市中心医院诊治的93例ccRCC患者癌组织中miR-4313、TBX15的mRNA的表达,免疫组织化学法检测癌组织中TBX15蛋白表达,以癌旁正常组织为对照。分析癌组织中miR-4313、TBX15蛋白表达与临床病理参数关系。Pearson线性相关分析miR-4313与TBX15 mRNA表达的相关性。结果与癌旁组织比较,ccRCC癌组织中miR-4313相对表达量明显较低,而TBX15 mRNA相对表达量明显较高,差异均有统计学意义(均P<0.05)。癌组织中TBX15蛋白表达阳性率明显高于癌旁组织,差异有统计学意义(P<0.05)。癌组织中miR-4313、TBX15蛋白表达在患者不同年龄、性别、病理分级、远处转移及淋巴结转移中表达比较差异无统计学意义(P>0.05)。肿瘤分期Ⅲ~Ⅳ期ccRCC癌组织中miR-4313表达明显低于Ⅰ~Ⅱ期癌组织,肿瘤分期Ⅲ~Ⅳ期ccRCC癌组织中TBX15蛋白阳性率明显高于Ⅰ~Ⅱ期癌组织,差异均有统计学意义(均P<0.05)。癌组织中miR-4313与TBX15 mRNA表达呈显著负相关(r=-0.671,P=0.001)。结论ccRCC癌组织中miR-4313表达降低,而TBX15表达升高,两者表达与肿瘤分期有关,有可能成为新的ccRCC肿瘤标志物。
Objective To study the expression and clinical significance of microRNA(miR)-4313 and T-box transcription factor 15(TBX15)in clear cell renal cell carcinoma(ccRCC).Methods Fluorescence quantitative PCR was used to detect the mRNA expression of miR-4313 and TBX15 in the cancer tissues of 93 ccRCC patients treated in Benxi Central Hospital,Liaoning Province from March 2018 to December 2019.Immunohistochemistry was used to detect the expression of TBX15 protein in the cancer tissues.Normal tissues adjacent to the cancer were used as control.The relationship between miR-4313 and TBX15 protein expression in cancer tissues and clinicopathological parameters was analyzed.The correlation between miR-4313 and TBX15 mRNA expression were analyzed by Pearson linear correlation analysis.Results Compared with adjacent tissues,the relative expression level of miR-4313 in ccRCC cancer tissue was significantly lower,while the relative expression level of TBX15 mRNA was significantly higher,and the differences were statistically significant(all P<0.05).The positive expression rate of TBX15 protein in cancer tissues was significantly higher than that in adjacent tissues,and the difference was statistically significant(P<0.05).There was no statistical significance in the expression of miR-4313 and TBX15 protein in cancer tissues among patients with different ages,genders,pathological grades,distant metastasis and lymph node metastasis(P>0.05).The miR-4313 expression in tumor stageⅢ-ⅣccRCC cancer tissues were significantly lower than stageⅠ-Ⅱ,the TBX15 protein expression in tumor stageⅢ-ⅣccRCC cancer tissues were significantly higher than the stageⅠ-Ⅱ,the differences were statistically significant(all P<0.05).There was a significant negative correlation between miR-4313 and TBX15 mRNA expression in cancer tissues(r=-0.671,P=0.001).Conclusion The expression of miR-4313 in ccRCC cancer tissues decreases,while the expression of TBX15 increases.Both expressions are related to tumor stage and may become new ccRCC tumor markers.
作者
苏计
王刚
冯世伟
SU Ji;WANG Gang;FENG Shiwei(Urinary Surgery,Benxi Central Hospital,Liaoning Province,Benxi117000,China)
出处
《中国医药导报》
CAS
2021年第10期19-22,F0003,共5页
China Medical Herald
基金
辽宁省卫生计生委科研课题(201703048)。
