摘要
于锰终浓度分别为0.0(对照组)、0.2、0.6、1.0、1.4 mmol/L的Hoagland营养液中培养芦竹(Arundo donax)幼苗,在Mn处理后8、16、24、32 d取样,测定芦竹叶的生理生化指标;在Mn处理后47、94 d分别测定芦竹叶的光合特性指标,探讨芦竹对Mn的耐受性。结果表明:在32 d内,除24 d 0.2 mmol/L组外,试验组和对照组的叶绿素相对含量没有显著差异;Mn处理下,脯氨酸质量分数初期(8 d)显著高于对照的,16 d时下降明显,32 d时最低,除24 d 1.4 mmol/L组外,后3期各Mn处理组的脯氨酸质量分数与对照间的差异无统计学意义;丙二醛含量除24 d 0.6 mmol/L组的显著高于对照外,其余各时期各组间的差异均无统计学意义;相对外渗率在前3期各组间的差异无统计学意义,但在32 d时,除0.6 mmol/L组外,其余各组的相对外渗率均显著高于对照的;与对照相比,后2期,1.4 mmol/L Mn处理会显著抑制超氧化物歧化酶活性和增强过氧化氢酶活性,对过氧化物酶活性除在16 d时有显著抑制外,其余各期影响不显著;Mn处理后47、94 d时,2次测定的净光合速率都随着锰离子浓度的增加而下降,但最低时仍维持在6.5μmol/(m^(2)·s);在96 d的试验期内,植株未表现出明显的锰毒症状。可见,芦竹对锰胁迫具备一定的耐性,可考虑作为锰矿尾矿渣和废弃地植物生态修复的优选物种。
We studied the effects of manganese stress on Arundo donax.The Hoagland nutrient solution served Mn provider agent by treating seedlings with final Mn concentrations of 0.0(control group),0.2,0.6,1.0,1.4 mmol/L.The physiological and biochemical components of giant reed leaves were measured at 8,16,24,32 d after Mn treatment,and photosynthetic characteristics of giant reed leaves were measured at 47,94 d after Mn treatment to explore its tolerance to manganese.The results showed that no significant changes were detected in the chlorophyll content among the treatments and the control except for the case of 0.2 mmol/L at 24 d.The content of proline was significantly higher than that of the control in the early stage(8 d),then decreased(16 d),and was the lowest at 32 d,with no significant differences among the treatments and control in the latter three stages except for the datum of 1.4 mmol/L at 24 d.No significant differences were observed in the MDA content except for the case of 0.6 mmol/L at 24 d.Though no differences were displayed in the relative exosmosis rate among the groups in the former stages of the treatment,the values increased significantly at 32 d when compared with the control except for that of 0.6 mmol/L treatment.Compared with the control,the activities of SOD were inhibited significantly and the activities of CAT were enhanced under 1.4 mmol/L Mn in the latter two stages.However,Mn treatment imposed little influence on the activity of POD through the experimental duration except for distinct inhibition at 16 d.Both the values of net photosynthetic rate tested at 47,94 d decreased obviously with the increase of Mn concentration,till the lowest 6.5μmol/(m^(2)·s).The seedlings presented no symptoms of manganese toxicity during 96 d,indicating giant reed had certain ability of tolerance to Mn stress,which made it possible to be considered as a potential crop for ecological restoration of manganese ore wastelands.
作者
赵发法
罗准
彭小列
易浪波
周强
揭雨成
刘世彪
ZHAO Fafa;LUO Zhun;PENG Xiaolie;YI Langbo;ZHOU Qiang;JIE Yucheng;LIU Shibiao(College of Biological Resources and Environmental Sciences,Jishou University,Jishou,Hunan 416000,China;Institute of Ramie,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第2期212-218,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(31760044、31872877)
生态旅游湖南省重点实验室开放基金项目(STLV1904)。
关键词
芦竹
锰胁迫
重金属
生理生化特性
光合特性
耐受性
giant reed(Arundo donax)
manganese stress
heavy metal
physiological and biochemical characteristics
photosynthesis feature
tolerance
