摘要
目的探究退变腰椎间盘髓核组织中酪氨酸激酶受体B(tyrosine kinase receptor B,TrkB)、N-Myc下游调节基因2(N-myc downstream regulatory gene 2,NDRG2)表达变化,并分析两者与衰老髓核细胞之间的相关性。方法选取2018年1月~2020年4月于本院行腰椎手术摘除的髓核组织标本75份,依据髓核退变程度Pfirrmann分级,划分为Ⅱ级组、Ⅲ级组、Ⅳ级组,各25例。采用细胞衰老β-半乳糖苷酶(senescence-associatedβ-galactosidase,SA-β-gal)试验检测髓核组织中衰老髓核细胞占比,采用RT-PCR技术检测微小RNA-200c(microRNA-200c,miR-200c)、TrkB mRNA及NDRG2 mRNA、P53 mRNA相对表达量,采用Western blotting法测定TrkB、NDRG2蛋白表达,并采用Pearson相关性分析TrkB、NDRG2表达与衰老髓核细胞的关系。结果三组髓核组织中的衰老髓核细胞百分比比较,存在显著差异(F=145.315,P<0.001)。三组miR-200c、TrkB mRNA、TrkB蛋白相对表达量比较,存在显著差异(P<0.05)。三组P53 mRNA、NDRG2 mRNA与NDRG2蛋白相对表达量比较,Ⅱ级组<Ⅲ级组<Ⅳ级组,差异均有统计学意义(P<0.05)。Pearson相关性分析显示,髓核组织中髓核细胞占比与TrkB mRNA相对表达量、TrkB蛋白相对表达量呈显著负相关(P<0.05),与NDRG2 mRNA相对表达量、NDRG2蛋白相对表达量呈显著正相关(P<0.05);miR-200c与TrkB mRNA呈显著负相关(r=-0.792,P<0.05),P53 mRNA与NDRG2 mRNA呈显著正相关(r=0.974,P<0.05)。结论TrkB、NDRG2可参与腰椎间盘退变病理过程,或经介导腰椎间盘髓核细胞衰老,促使椎间盘退变。
Objective To explore the expression changes of tyrosine kinase receptor B(TrkB)and N-Myc downstream regulatory gene 2(NDRG2)in the degenerated lumbar intervertebral disc nucleus pulposus tissue,and to analyze the correlation between them and senescent nucleus pulposus cells.Methods A total of 75 specimens of nucleus pulposus tissues removed from lumbar spine surgery in our hospital from January 2018 to April 2020 were selected.According to the Pfirrmann grading of nucleus pulposus degeneration,they were divided into grade II,grade III,and grade IV groups,with 25 examples in each group.Cell senescenceβ-galactosidase(SA-β-gal)test was used to detect the proportion of senescent nucleus pulposus cells in nucleus pulposus tissue,and RT-PCR technology was used to detect the relative expression of microRNA-200c(miR-200c),TrkB mRNA,NDRG2 mRNA and P53 mRNA.Western blotting was used to determine the expression of TrkB,NDRG2 protein,and Pearson correlation was used to analyze the relationship between TrkB,NDRG2 expression and senescent nucleus pulposus cells.Results There were significant differences in the percentage of senescent nucleus pulposus cells among three groups of nucleus pulposus tissues(F=145.315,P<0.001).The relative expression of miR-200c,TrkB mRNA and TrkB protein among three groups were significantly different(P<0.05).The relative expression levels of P53 mRNA,NDRG2 mRNA and NDRG2 protein among three groups were significantly different,and those in the gradeⅡgroup<gradeⅢgroup<gradeⅣgroup(P<0.05).Pearson correlation analysis showed that the proportion of nucleus pulposus cells in the nucleus pulposus tissue was negatively correlated with the relative expression of TrkB mRNA and TrkB protein(P<0.05),and was positively correlated with the relative expression of NDRG2 mRNA and NDRG2 protein(P<0.05).miR-200c was significantly negatively correlated with TrkB mRNA(r=-0.792,P<0.05),and P53 mRNA was significantly positively correlated with NDRG2 mRNA(r=0.974,P<0.05).Conclusion TrkB and NDRG2 can participate in the pathological process of lumbar intervertebral disc degeneration,or mediate the senescence of lumbar intervertebral disc nucleus pulposus cells to promote the degeneration of the intervertebral disc.
作者
蔡正生
孙竹清
杨晓松
赵宏宇
郑基永
CAI Zheng-sheng;SUN Zhu-qing;YANG Xiao-song;ZHAO Hong-yu;ZHENG Ji-yong(Department of General Surgery,Shenyang Orthopedic Hospital,Shenyang,Liaoning,110000,China)
出处
《颈腰痛杂志》
2021年第2期164-168,218,共6页
The Journal of Cervicodynia and Lumbodynia
基金
宁夏回族自治区自然科学基金(编号:NZ16212)。
