摘要
可降解材料作为生物材料的重要组成部分,其体内降解性能的好坏往往决定着材料植入后的成败。因此,对材料体内降解的评价显得尤为重要。传统的生物材料体内降解评价方法需要在各取样点取出不同批次的降解样品,阻止了对同一个实验样品降解过程的连续测量,并且存在样品需求量大的问题。小动物活体成像系统(in vivo imaging system,IVIS)具有非侵入性、操作性强等特点,为解决上述问题提供了思路。本研究旨在建立一种利用小动物荧光成像系统检测可降解材料降解性能的方法,通过将近红外荧光染料经化学反应标记到可降解材料上,由荧光强度的变化反应材料的降解程度。体内降解实验表明此方法制得的荧光标记材料,荧光稳定性高,材料降解过程中荧光强度变化与质量损失拟合效果良好(R2=0.9994)。综上,该方法解决了测量材料降解样品量大的问题,并且提高了实验过程的连贯性。
Degradable material is an important part of biomaterials, its degradation performance in vivo has great influence on the final success after implantation. Thus, the method of evaluating the degradation of the materials in vivo is crucial to the evaluation of material performance. The traditional method to estimate the degradation in vivo needs to take out different batches of degradation samples at each sampling point, which prevents the continuous measurement of the degradation process and it needs a large number of samples. In vivo imaging system(IVIS) has the characteristics of non-invasive and strong operability, which provides a way to solve the problems above. In this study, we established a method to detect the degradation performance of degradable materials with IVIS. The research method was that the near-infrared fluorescent dye was labeled on the degradable materials by chemical reaction, and then using the change of fluorescence intensity to reflect the degradation degree of the materials. The in vivo degradation experimental results show that the fluorescent labeling materials prepared by this method have high fluorescence stability, and the fitting effect of fluorescence intensity and mass loss in the process of material degradation is good(R2 = 0.9994). In conclusion, this method solves the problem of large amount of samples in the traditional method of measuring material degradation, and improves the continuity of the experimental process.
作者
刘秀芸
陈丹藜
关淇峰
刘田
杨丰合
牛旭锋
LIU Xiuyun;CHEN Danli;GUAN Qifeng;LIU Tian;YANG Fenghe;NIU Xufeng(School of Biological Science and Medical Engineering,Beihang University,Beijing 100083,China;Beijing Advanced Innovation Center for Biomedical Engineering,Beihang University,Beijing 100083,China)
出处
《复合材料学报》
EI
CAS
CSCD
北大核心
2021年第2期487-495,共9页
Acta Materiae Compositae Sinica
基金
国家自然科学基金(11872097)
北京市自然科学基金-海淀原始创新联合基金(L182017)。
关键词
可降解医用高分子材料
体内降解
小动物活体成像系统
荧光标记
评价方法
degradable medical polymer materials
in vivo degradation
in vivo imaging system
fluorescence labeling
evaluation method
