摘要
目的:探究姜黄素对肺癌相关长链非编码RNA(LncRNA)的表达及厄洛替尼敏感性的影响。方法:以20μmol·L^(-1)姜黄素处理非小细胞肺癌(NSCLC)PC9亲本细胞株作为实验组,以0.9%氯化钠溶液处理的厄洛替尼抵抗PC9细胞株为抵抗组,20μmol·L^(-1)姜黄素处理的厄洛替尼抵抗PC9细胞株为处理组,0.9%氯化钠溶液处理的PC9亲本细胞株为对照组。实时荧光定量PCR(qPCR)检测各组细胞中LncRNA MALAT1、LSINCT5、UCA1、HOTAIR、GAS5及H19表达差异,CCK-8实验检测各组细胞厄洛替尼半数抑制浓度(IC50)的差异。结果:相比对照组,实验组细胞MALAT1、HOTAIR、LSINCT5及H19表达均显著降低(P<0.05),UCA1及GAS5表达差异无统计学意义(P>0.05),厄洛替尼IC50差异无统计学意义(P>0.05);相比对照组,抵抗组及处理组各LncRNA及厄洛替尼IC50均显著增高(P<0.05);相比抵抗组,处理组LncRNA MALAT1、LSINCT5、HOTAIR、GAS5及H19表达均显著降低,且厄洛替尼IC50下降(P<0.05)。结论:姜黄素可调控EGFR-TKI抵抗肺癌细胞中多种相关LncRNA的表达,促进抵抗细胞对厄洛替尼的敏感性。
Objective:To investigate the effect of curcumin on the expression of lung cancer-related long non-coding RNA(LncRNA)and its sensitivity to erlotinib.Methods:PC9 parent cell lines that belonged to nonsmall cell lung cancer(NSCLC)with EGFR mutation were treated with 20μmol·L^(-1)curcumin as the experimental group.Erlotinib-resistant PC9 cell lines treated with 0.9%sodium chloride,Erlotinib-resistant PC9 cell lines treated with 20μmol·L^(-1) curcumin and PC9 parent cell lines treated with 0.9%sodium chloride were used respectively as the resistance group,treatment group and control group.The expression of LncRNA MALAT1,LSINCT5,UCA1,HOTAIR,GAS5 and H19 were detected by real-time fluorescence quantitative PCR(qPCR),and the Erlotinib semi-inhibitory concentrations(IC50)of each group cells were detected by CCK-8 assay.Results:Compared with the control group,the expressions of MALAT1,HOTAIR,LSINCT5 and H19 in the experimental group decreased significantly(P<0.05),while there was no significant difference in the expressions of UCA1 and GAS5 and IC50 to Erlotinib(P>0.05).Compared with the control group,the expression of each LncRNA and IC50 to Erlotinib in the resistance group and the treatment group increased significantly(P<0.05).Compared with the resistance group,the expression of LncRNA MALAT1,LSINCT5,HOTAIR,GAS5 and H19 and IC50 to Erlotinib decreased significantly in the treatment group(P<0.05).Conclusion:Curcumin can regulate the expression of various LncRNA related to EGFR-TKI resistance in lung cancer cells and promote the sensitivity of resistance cells to erlotinib.
作者
徐亚莉
曹晓静
朱小珍
朱光辉
XU Yali;CAO Xiaojing;ZHU Xiaozhen;ZHU Guanghui(Department of Pharmacy,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2021年第3期225-229,共5页
Journal of Wenzhou Medical University
