摘要
目的:探究环状RNAs(circRNAs)在氧化型低密度脂蛋白(ox-LDL)刺激的巨噬细胞中的差异表达情况及功能预测。方法:采用人单核细胞系THP-1,用佛波醇12-十四酸酯13-乙酸酯(PMA)预处理细胞48 h,随后给予ox-LDL 50μg/mL刺激单核源巨噬细胞24 h构建巨噬细胞炎症模型。利用microarray芯片筛选出差异表达的circRNAs。通过qRT-PCR实验检测芯片中差异表达的circRNAs。利用CircInteractome、TargetScan和miRecords数据库预测circRNA_0007478潜在的靶基因。结果:芯片筛选结果提示在ox-LDL刺激的巨噬细胞中有5条circRNAs表达显著上调,2条circRNAs表达显著下调。qRT-PCR证实其中4条表达显著上调,2条表达显著下调。生物信息学分析推测,circRNA_0007478可通过结合miRNA-765进而影响下游蛋白的表达,发挥潜在的调控作用。结论:在ox-LDL刺激的巨噬细胞中存在circRNAs的差异性表达,circRNA_0007478可能通过结合miRNA-765调控下游基因参与动脉粥样硬化进程。
Objective:To investigate the differential expression profiles of circRNAs in ox-LDL-stimulated macrophages and predict its functions.Methods:Human monocyte cell line THP-1 cells were pretreated with PMA for 48 h and followed by ox-LDL(50μg/mL)for 24 h to establish cell model.Microarray analysis was performed to investigate the differential expression profiles of circRNAs in macrophages with or without ox-LDL stimulation(as a control).qRT-PCR was utilized to verify the content variation of circRNAs in macrophages with ox-LDL stimulation.CircInteractome,TargetScan and miRecords websites were used to predict potential target genes.Results:There were 5 upregulated circRNAs and 2 downregulated circRNAs in ox-LDL-stimulated macrophages group,compared with the control group in the present study.We also verified that there were 4 circRNAs upregulated and 2 circRNAs downregulated in macrophages with ox-LDL stimulation by qRT-PCR.Bioinformatics analysis speculated that circRNA_0007478 could affect the expression of downstream proteins by binding to miR-765.Conclusion:There exist differential expressions of circRNAs in ox-LDL-stimulated macrophages.CircRNA_0007478 is predicted to be involved in the atherosclerosis process by binding to miRNA-765 to regulate downstream genes.
作者
叶浡之
梁晓荷
赵翊含
薛淇泽
黄周青
蔡雪黎
YE Bozhi;LIANG Xiaohe;ZHAO Yihan;XUE Qize;HUANG Zhouqing;CAI Xueli(Department of Cardiology,the Key Lab of Cardiovascular Disease of Wenzhou,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;The Second School of Medicine,Wenzhou Medical University.Wenzhou 325035,China)
出处
《温州医科大学学报》
CAS
2021年第3期203-208,共6页
Journal of Wenzhou Medical University
基金
温州市科技计划项目(Y20170045)。
