摘要
为了解nirS和nirK型反硝化细菌在水稻根中的分布,利用MiSeq测序平台,对水稻根部的nirS和nirK反硝化功能基因进行高通量测序,并对测得序列进行生物信息学分析,揭示nirS和nirK型反硝化细菌在水稻根中的分布特征。结果表明:nirK型反硝化细菌的种类丰富度虽高于nirS型,但种类分布的均匀度却低于nirS型。nirS和nirK型反硝化细菌隶属于3门5纲12目26属。在门水平,变形菌门是nirS和nirK型反硝化细菌的共同优势门。在纲水平,α-变形菌纲是nirS和nirK型反硝化细菌的共同优势纲。在目水平,红螺菌目是nirS型反硝化细菌的优势目;根瘤菌目是nirK型反硝化细菌的优势目。在属水平,磁螺菌属是nirS型反硝化细菌的优势属;氏菌属、红假单胞菌属、慢生根瘤菌属是nirK型反硝化细菌的优势属。nirS和nirK型反硝化细菌在水稻根中的分布特征与前人对其他地理环境的同类研究结果相一致。固氮螺菌属等5个类群细菌仅存在于nirS型反硝化细菌中;氏菌属等17种类群细菌仅存在于nirK型反硝化细菌中,呈现出分布特异性。
In order to understand the distribution of nirS and nirK denitrifying bacteria in rice roots.High-throughput sequencing of nirS and nirK denitrification functional genes in rice roots was carried out by using MiSeq sequencing platform.Bioinformatics analysis was carried out to reveal the distribution characteristics of nirS and nirK denitrifying bacteria in rice roots.The results showed that although the species richness of nirK-type denitrifying bacteria was higher than that of nirS-type denitrifying bacteria,the species distribution uniformity of nirK-type denitrifying bacteria was lower than that of nirS-type denitrifying bacteria.nirS and nirK denitrifying bacteria belong to 26 genera,12 orders and 3 phyla,5 classes.At the phylum level,proteobacteria is the common predominant phylum of nirS and nirK denitrifying bacteria.At the class level,alphaproteobacteria is the common dominant class of nirS and nirK denitrifying bacteria.Rhizobium is the dominant order of nirS denitrifying bacteria and Rhizobium is the dominant order of nirK denitrifying bacteria.At genus level,magnetospirillum is the dominant genus of nirStype denitrifying bacteria,while bosea,rhodopseudomonas,bradyrhizobium are the dominant genus of nirK-type denitrifying bacteria.The distribution characteristics of nirS and nirK denitrifying bacteria in rice roots ars consistent with the results of similar studies in other geographical environments.Five groups of bacteria such as Azospira existed only in nirS denitrifying bacteria,and 17 groups of bacteria such as Bosea existed only in nirK denitrifying bacteria,showing a special distribution.
作者
陈泽斌
李冰
林丽
刘佳妮
余磊
徐胜光
CHEN Zebin;LI Bing;LIN Li;LIU Jiani;YU Lei;XU Shengguang(College of Agronomy and Life Sciences,Kunming University,Kunming 650214;Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223;Engineering Research Center for Characteristics Agriculture of Yunnan Province,Kunming 650214;Engineering Research Center for Biochar of High Education of Yunnan Prov-ince,Kunming 650214,China)
出处
《东北农业科学》
2021年第1期62-67,共6页
Journal of Northeast Agricultural Sciences
基金
国家自然科学基金项目(42067009)
云南省地方本科高校基础研究联合专项(2017FH001-041、2017FH001-042)。
