摘要
为了提高常规籼稻品种‘黄华占’和‘五山丝苗’的褐飞虱抗性,采用杂交、回交和分子标记辅助选择技术,将褐飞虱抗性基因Bph14和Bph15分别导入到‘黄华占’和‘五山丝苗’的遗传背景中,选育出了具有‘黄华占’和‘五山丝苗’遗传背景,同时携带纯合Bph14和Bph15基因的新株系各2个。苗期褐飞虱抗性鉴定结果表明,选出的新株系均表现为抗褐飞虱。产量比较试验、性状考查和米质分析结果表明,新株系的产量、主要性状和稻米品质指标都与受体亲本相似,因此可以作为‘黄华占’和‘五山丝苗’的抗褐飞虱新品系进一步进行多点试验和区域试验。
In order to improve the resistance of Huanghuazhan(HHZ)and Wushansimiao(WSSM)to brown planthopper(BPH),two BPH-resistant genes,Bph14 and Bph15,were successfully pyramided into the genetic background of HHZ and WSSM by crossing,backcrossing and molecular marker-assisted selection(MAS),respectively.Two improved lines with genetic background of each HHZ and WSSM carried homozygous Bph14 and Bph15 genes were developed.BPH bioassays results showed that the improved lines were all resistant to BPH at the seedling stage.The field yield test,major agronomic traits evaluation and grain quality analysis results showed that the improved lines were basically identical to the corresponding recipient.These improved lines could be used as new BPH-resistant strains for further multi-point and regional tests.
作者
毛方明
王红波
牟同敏
MAO Fang-ming;WANG Hong-bo;MOU Tong-min(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;Jiangxi Super Rice Research and Development Center,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《江西农业学报》
CAS
2021年第3期11-16,共6页
Acta Agriculturae Jiangxi
基金
国家重点研发计划(2016YFD0101104)
湖北省技术创新专项(2018ABA083)。
关键词
水稻
褐飞虱
抗性
分子标记辅助选择
改良
Rice
Brown planthopper
Resistance
Molecular marker-assisted selection
Improvement
