摘要
目的研究左金丸醇提物对人胃癌细胞SGC-7901增殖和糖酵解的作用及机制。方法采用CCK-8法检测左金丸醇提物(5、10、25、50、100、200μg/mL)对SGC-7901细胞增殖的影响;检测左金丸醇提物对SGC-7901细胞葡萄糖摄取量、乳酸和三磷酸腺苷(adenosine triphosphate,ATP)含量的影响,评价左金丸醇提物对SGC-7901细胞糖酵解的作用;采用qRT-PCR和Western blotting法检测左金丸醇提物对细胞糖酵解途径相关蛋白Rhotekin(RTKN)表达的影响;采用RTKN shRNA干扰慢病毒感染SGC-7901细胞,检测左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的影响。结果左金丸醇提物对SGC-7901细胞增殖具有显著抑制作用(P<0.001),呈剂量、时间相关性;左金丸醇提物显著降低SGC-7901细胞葡萄糖摄取、乳酸和ATP含量(P<0.01、0.001);左金丸醇提物显著降低SGC-7901细胞中RTKN mRNA和RTKN蛋白表达(P<0.001);干扰RTKN抵消了左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的抑制作用(P<0.001)。结论左金丸醇提物可显著抑制SGC-7901细胞增殖、降低细胞糖酵解水平,其机制与下调RTKN表达相关。
Objective To investigate the effect of Zuojin Pills(左金丸)alcohol extract on proliferation and glycolysis of gastric cancer SGC-7901 cells by downregulating Rhotekin(RTKN).Methods CCK-8 was used to evaluate the effect of Zuojin Pills alcohol extract(10,25,50,100,200μg/m L)on cell viability of SGC-7901 cell.Glucose uptake,lactate and ATP content of SGC-7901 cells were detected to evaluate the effects of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells.qRT-PCR and Western blotting were used to detect glycolysis related protein RTKN expression.Moreover,RTKN sh RNA were transfected into SGC-7901 cells to knockdown RTKN and the effects of Zuojin Pills alcohol extract on glucose uptake,lactate and ATP content were evaluated.Results Zuojin Pills alcohol extract exhibited a dose-and time-dependent anti-proliferative effect on SGC-7901 cells(P<0.001).In addition,glucose uptake,lactate and ATP content of SGC-7901 cells were significantly decreased(P<0.01,0.001)with Zuojin Pills alcohol extract treatment.Zuojin Pills alcohol extract significantly reduced RTKN m RNA and RTKN protein expression in SGC-7901 cells(P<0.001).However,RTKN knockdown significantly attenuated the inhibitory effect of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells(P<0.001).Conclusion Zuojin Pills alcohol extract significantly inhibited the proliferation and glycolysis of SGC-7901 cells,which was related to the downregulation of RTKN.
作者
吴秋雪
孙梦瑶
许博
张辉
汤庆丰
WU Qiu-xue;SUN Meng-yao;XU Bo;ZHANG Hui;TANG Qing-feng(Putuo Hospital,shanghai University of Traditional Chinese Medicine,Shanghai 200062,China;Shanghai University of Traditional Chinese Medicine,Shanghai 200135,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第1期145-151,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81973808)
上海市自然科学基金资助项目(19ZR1447800)
上海中医药大学预算内项目(2019LK038)。
