摘要
为了解贵州省规模化鸡场鸡白痢流行现状,2017—2019年选取31个规模化鸡场抽检1340份鸡血清样品,采用鸡白痢抗体平板凝集试验,结合鸡场生物安全措施及净化情况,开展了鸡白痢血清学调查。结果显示:鸡白痢平均场群阳性率为83.87%,个体阳性率为13.43%;商品场(15.24%)鸡白痢个体阳性率最高,与父母代鸡场差异显著(P=0.044);育成期(18.77%)个体阳性率高于产蛋期(11.83%),差异显著(P=0.002);不同饮水来源、不同饲养方式的鸡白痢个体阳性率间有差异,但不显著(P>0.05)。车辆人员进出场区时消毒、空舍消毒的鸡白痢个体阳性率较低,但不显著(P>0.05),消毒频率越高,阳性率越低;31个调查场点中,引种前开展疫病检测及采取鸡白痢净化控制措施的场点个体阳性率低于未开展净化的场点,差异极显著(P=0.001、<0.001);仅有32.26%(10/31)场点有意愿开展鸡白痢净化。结果表明:贵州省规模鸡场鸡白痢沙门氏菌感染较为普遍,污染面较广;其感染受场点类型、生长阶段影响较大,而受饲养方式和饮水来源影响较小。结果提示:鸡场实施鸡白痢净化时,可以着重考虑场点类型和生长阶段这两个因素;合理设置场点地理位置、加强场点消毒和开展鸡白痢净化,可显著降低鸡白痢流行率,因此应加强宣传,提高规模化鸡场的鸡白痢防控及净化意识,主动开展鸡白痢净化工作,以控制鸡白痢流行。
In order to investigate the prevalence status of pullorum disease in some large-scale chicken farms in Guizhou Province,1340 serum samples were collected from 31 farms from 2017 to 2019 to carry out a serological investigation for pullorum disease by the plate agglutination test and in combination with biosecurity measures and purification status in the farms.The results showed that the average positive rate at farm/population level was 83.87%,and that at the individual level was 14.43%;the individual positive rate of commercial farms was highest(15.24%),which was quite different from that of parents'generation farms(P=0.044);the individual positive rate was higher during growing stage(18.77%)than that during egg laying stage(11.83%),with obvious difference(P=0.002);the individual positive rate was different in various drinking water sources and feeding methods with not significant difference(P>0.05);the individual positive rate was relatively low when vehicles and personnel were disinfected as they were leaving or arriving or empty henhouses were disinfected,but the difference was not significant(P>0.05),and the positive rate decreased with the increasing disinfection frequency;the individual positive rate of the premises where surveillance and purification measures were taken prior to introduction of chicken was lower than that of unpurified premises,with extremely significant difference(P=0.001,<0.001);only 32.26%(10/31)premises were willing to carry out the purification.It was concluded that the infection with Salmonella pullorum was widely distributed in large-scale farms in Guizhou Province,which was mostly affected by the type of premises and growth stage compared with feeding methods and drinking water sources.Therefore,the type of premises and growth stage should be emphatically considered when farms were purified against pullorum disease;the prevalence rate could be reduced by reasonable location of premises,strengthening disinfection and purification in premises,so publicity activities should be carried out to improve the awareness for prevention,control and purification of pullorum disease in large-scale farms,and thus to carry out purification to control the disease.
作者
李照伟
李涛
蒙炳超
王慧
何菊
张海
王素春
Li Zhaowei;Li Tao;Meng Bingchao;Wang Hui;He ju;Zhang Hai;Wang Suchun(Guizhou Animal Disease Prevention and Control Center,Guiyang,Guizhou 550008,China;Qiannan Buyi and Miao Autonomous Prefecture Livestock Industry Development Center,Duyun,Guizhou 558000,China;Shuicheng Animal Disease Prevention and Control Center,Shuicheng,Guizhou 553000,China;Guiyang Animal Disease Prevention and Control Center,Guiyang,Guizhou 550081,China;China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China)
出处
《中国动物检疫》
CAS
2021年第3期9-12,共4页
China Animal Health Inspection
关键词
鸡白痢
血清学调查
生物安全
贵州省
pullorum disease
serological investigation
bio-security
Guizhou Province
