摘要
揭示热休克蛋白27(Heat Shock Protein 27,HSP27)可通过参与机体的氧化应激过程从而影响奶牛RFM发生的具体机制。采集奶牛胎衣不下(RFM)组及胎衣正常排出母体组的胎盘组织,应用WB及试剂盒检测HSP27的相对表达以及葡萄糖6磷酸脱氢酶(G6PD)、谷胱甘肽过氧化物酶(GSH-Px)活性;还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)含量变化。在建立奶牛子宫内膜上皮细胞(UCE)氧化应激模型的基础之上,使用17-AAG对HSP27的表达进行过表达,检测上述指标的变化。HSP27在RFM组母体胎盘组织中的表达量显著高于胎衣正常排出(P<0.05),大多以磷酸化形式存在,RFM奶牛G6PD、GSH-Px活性以及GSH含量都显著低于胎衣正常排出组奶牛(P<0.05),RFM组GSSG含量显著高于胎衣正常奶牛(P<0.05)。在UCE中,HSP27过表达组G6PD、GSH-Px活性以及GSH含量都显著高于于对照组(P<0.05),HSP27过表达组GSSG含量以及ROS含量都显著低于对照组(P<0.05)。HSP27通过上调细胞内G6PD、GSH-Px活性进而上调GSH含量减轻ROS对细胞造成的损伤,进而影响胎衣排出。
To investigate the regulation of heat shock protein 27(HSP27)on oxidative stress in cows with and without retained fetal membranes(RFM),the relative expression of HSP27,the activity of glucose 6 phosphate dehydrogenase(G6PD)and glutathione peroxidase(GSH-Px),and the expression levels of reduced glutathione(GSH)and oxidized glutathione(GSSG)were detected in the placental tissues of RFM group and control group.The bovine uterine caruncle epithelial cells(UCE)oxidative stress model was established through the preliminary experiment,and the changes of the above indicators were detected through the activation of HSP27.The relative expression of HSP27 in the placenta of RFM cows was significantly higher than that of normal cows with placenta(P<0.05),but most of them existed in phosphorylation form.The G6PD and GSH-Px activity and GSH content of RFM cows were significantly lower than that of normal cows(P<0.05),while the GSSG content of RFM cows were significantly higher than that of normal cows(P<0.05).In UCE,G6PD and GSH-Px activity and GSH content in the HSP27 activation group were significantly higher than that in control group(P<0.05),while the GSSG and ROS content in the HSP27 activation group were significantly lower than that in control group(P<0.05).HSP27 reduces the damage of ROS to cells by up-regulating the activity of G6PD and GSH-Px in the cells and then up-regulating the content of GSH,thereby affecting the discharge of fetal clothing.
作者
张梦龙
赵璧忱
邢菲菲
罗胜缤
罗春海
付世新
Zhang Menglong;Zhao Bichen;Xing Feifei;Luo Shengbin;Luo Chunhai;Fu Shixin(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2021年第1期21-26,75,共7页
journal of heilongjiang bayi agricultural university
基金
黑龙江省农垦总局重点科研项目(HKKY190302)。
