摘要
目的检测调节Th1/Th2细胞分化的T-bet,GATA3蛋白在不同程度获得性再生障碍性贫血患儿骨髓中的表达,同时比较其治疗前外周血IL-2、IL-4、IFN-γ细胞因子水平,探讨获得性再生障碍性贫血的免疫病理机制。方法将49例获得性再生障碍性贫血患儿根据临床表现分为非重型组和重型与极重型组。利用免疫组织化学方法检测骨髓病理切片中T-bet,GATA3蛋白表达。酶联免疫吸附法比较细胞因子IL-2、IL-4、IFN-γ水平。结果T-bet在重型与极重型组AOD值0.0170(0.0140~0.0210)显著高于非重型组0.0060(0.0051~0.0064),P=0.000。GATA3在重型与极重型组和非重型组表达差异无统计学意义(0.0053±0.0010,0.0052±0.0009,P=0.846)。IL-2及IFN-γ在重型与极重型组显著高于非重型组(P=0.000,P=0.007),IL-4两组比较差异无统计学意义(P=0.289)。结论骨髓衰竭重的获得性再生障碍性贫血患儿其骨髓T-bet蛋白表达明显增高,外周血TH1细胞相关因子水平增高,调节Th1的T-bet蛋白在再生障碍性贫血的免疫病理机制中可能起着一定作用。
Objective To investigate the expression of transcription factors T-bet and GATA3 in bone marrow,and IL-2,IL-4 and IFN-γlevels in peripheral blood of children with acquired aplastic anemia.Methods Forty nine children with acquired aplastic anemia were enrolled in the study,including 25 non-severe cases(NSAA) group and 24 severe and very severe cases(SAA/VSAA group).Immunohistochemical staining was used to detect T-bet and GATA3 protein expressions in bone marrow pathological sections.Enzyme linked immunosorbent assay(ELISA)was used to determine serum levels of IL2,IL4 and IFN-γ.Results The AOD value of T-bet in SAA/VSAA group was 0.0170(0.0140-0.0210),which was significantly higher than that in NSAA group[0.0060(0.0051-0.0064),P=0.000].There was no significant difference in the expression of GATA3 between the two groups(P=0.846).Serum IL-2 and IFN-γlevels were significantly higher in the SAA/VSAA group than those in NSAA group(P=0.000,P=0.007).There was no significant difference in IL-4 levels between the two groups(P=0.289).Conclusion The expression of T-bet protein in the bone marrow of aplastic anemia patients with severe bone marrow failure is significantly increased,and the levels of Th1 cytokines in peripheral blood are also increased,indicating that T-bet protein regulating Th1 may play a role in the immunopathological mechanism of aplastic anemia.
作者
柯福彬
Anil Kumar Yadav
谢晓恬
乔晓红
KE Fu-bin;Anil KumarYadav;XIE Xiao-tian;QIAO Xiao-hong(Dept.of Pediatrics,Tongji Hospital,Tongji University School of Medicine,Shanghai 200065,China)
出处
《同济大学学报（医学版）》
CAS
2021年第1期80-84,共5页
Journal of Tongji University(Medical Science)
基金
国家自然科学基金面上项目(81670119)。
