摘要
以黑曲霉(Aspergillus niger)TL-F2为供试菌株,黑麦草为供试植物,研究Cd胁迫下A.niger TL-F2的促生特性,以及对黑麦草种子萌发、幼苗生长和Cd含量的影响。结果表明:与不加Cd的对照组相比,低浓度Cd(5 mg·L~(-1))胁迫下,A.niger TL-F2产吲哚乙酸(IAA)和溶磷的能力无显著变化,但产铁载体的能力显著(P<0.05)降低48.18%;中(20 mg·L~(-1))、高(50 mg·L~(-1))浓度Cd胁迫下,A.niger TL-F2产IAA的能力分别显著(P<0.05)降低55.76%和65.69%,溶磷量分别显著(P<0.05)降低50.07%和78.19%,产铁载体的能力分别显著(P<0.05)降低69.71%和80.08%。接种高浓度(1×10~8 mL~(-1))A.niger TL-F2有助于促进Cd胁迫下黑麦草种子的萌发和生长,高浓度Cd胁迫下,黑麦草地上部Cd含量较不接菌的显著(P<0.05)增加,增幅为17.95%;接种低浓度(1×10~6 mL~(-1))A.niger TL-F2对黑麦草种子萌发、Cd含量无明显促进作用。综合来看,接种适当量的A.niger TL-F2有助于促进Cd胁迫下黑麦草种子的萌发和生长,增加黑麦草地上部Cd含量,可作为黑麦草修复Cd污染水体和土壤的强化措施。
The growth-promoting characteristics of Aspergillus niger TL-F2,and its effects on seed germination,seedling growth and Cd content of ryegrass under Cd stress were studied.The results showed that the siderophore production of A.niger TL-F2 was significantly(P<0.05)decreased by 48.18%,and there was no significant change of indoleacetic acid(IAA)production and phosphate solubilization under low Cd(5 mg·L-1)stress.However,under medium(20 mg·L-1)and high(50 mg·L-1)Cd stress,the IAA production,phosphate solubilization and siderophore production of A.niger TL-F2 was significantly(P<0.05)decreased by 55.76%and 65.69%,50.07%and 78.19%,69.71%and 80.08%,respectively.Inoculation of 1×108 mL-1 A.niger TL-F2 could benefit the seed germination and seedling growth of ryegrass,and the Cd content in shoots was significantly(P<0.05)increased by 17.95%than that without inoculation under high Cd stress.However,inoculation of 1×106 mL-1 A.niger TL-F2 showed no significant effect on seed germination and seedling growth of ryegrass.The results suggested that inoculation of proper amount of A.niger TL-F2 could promote seed germination and seedling growth of ryegrass,and enhance the accumulation of Cd in the shoots of ryegrass seedlings.Therefore,inoculation of A.niger TL-F2 had the potential as a new means to enhance the remediation of Cd polluted soil and water by using ryegrass.
作者
刘如
董畅茹
张祎雯
屈铭慧
张伟
洒海洋
陈海燕
叶文玲
樊霆
LIU Ru;DONG Changru;ZHANG Yiwen;QU Minghui;ZHANG Wei;SA Haiyang;CHEN Haiyan;YE Wenling;FAN Ting(Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention,School of Resources and Environment,Anhui Agricultural University,Hefei 230036,China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第2期326-334,共9页
Acta Agriculturae Zhejiangensis
基金
国家重点研发计划(2019YFC1805203)
国家自然科学基金(41101485)
安徽省高等学校自然科学研究项目(K1952001)
安徽省农田生态保育与污染防控安徽省重点实验室开放基金(FECPP201903)
国家级大学生创新创业训练计划(202010364065)
安徽省大学生创新创业训练计划(201910364228)
安徽农业大学大学生创新基金(XJDC2019207,XJDC2020463)。
关键词
黑麦草
镉
黑曲霉
种子萌发
促生特性
ryegrass
cadmium
Aspergillus niger
seed germination
growth-promoting characteristics
