摘要
为探究p38 MAPK抑制剂SB203580对热应激雌性雏鸡主要生殖激素分泌的影响,将72只1日龄文昌鸡母雏随机分为对照组(CK)、热应激组(HS)和抑制剂组(HS+I),从第三周开始,每天中午12∶00对HS、HS+I组雏鸡进行热应激2 h,每周龄末在热应激前30 min给HS+I组雏鸡腹腔注射SB203580(5 mg/kg体重)。采用ELISA对血浆中促性腺激素释放激素(GnRH)、促卵泡素(FSH)、促黄体生成素(LH)、雌二醇(E2)的含量进行测定。结果显示:HS组GnRH含量在3周龄时比CK组显著上升,而在4、5、6周龄时显著降低,HS+I组在3、4周龄时比HS组显著降低(P<0.05);HS组FSH含量在3周龄比CK组显著增加而在6周龄显著减少,HS+I组在3周龄比HS组显著减少(P<0.05);HS组LH含量在3周龄比CK组显著上升而在4、6周龄显著下降,HS+I组在3周龄比HS组显著减少(P<0.05);HS组E2含量在3周龄比CK组显著上升而在5、6周龄显著下降,HS+I组在3周龄比HS组显著减少(P<0.05)。结果表明:热应激扰乱血浆中GnRH、FSH、LH、E2的正常水平,SB203580可抑制热应激雏鸡体内GnRH、FSH、LH、E2的分泌。
Seventy-two 1-day-age female Wenchang chicks were randomly divided into the control group(CK),the heat stress group(HS)and the inhibitor group(HS+I).Starting from the third week,the chicks in HS and HS+I were subjected to heat stress for 2 hours at 12∶00(noon)every day.At the end of each week,p38 MAPK inhibitor,SB203580(5 mg/kg BW)was intraperitoneally injected into chicks in the HS+I 30 minutes before heat stress.The contents of GnRH,FSH,LH and E2 in plasma were determined by ELISA.GnRH in the HS increased significantly at the 3 rd week than the CK,and decreased significantly at other weeks,and which of HS+I was significantly lower than the HS at3 rd and 4 th week(P<0.05).FSH in the HS increased significantly at the 3 rd week and decreased significantly at the6 th week than the CK,the HS+I decreased significantly at the 3 rd week than the HS(P<0.05).LH in the HS increased significantly at the 3 rd week than the CK,but decreased significantly at the 4 th and 6 th week,and which of HS+I decreased significantly at the 3 rd week than the HS(P<0.05).The E2 in the HS increased significantly at the3 rd week than the CK,but decreased significantly at the 5 th and 6 th week,and which of the HS+I decreased significantly at the 3 rd week than the HS(P<0.05).The results indicated that heat stress disturbed the normal levels of GnRH,FSH,LH and E2 in plasma,and SB203580 inhibited the secretion of GnRH,FSH,LH and E2 in chicks under heat stress.
作者
余哲琪
田佳迎
李启黉
陈忠
YU Zheqi;TIAN Jiaying;LI Qihong;CHEN Zhong(Ministry of Education Key Laboratory for Ecology of Tropical Islands,Hainan Normal University,Haikou,Hainan 571158)
出处
《中国家禽》
北大核心
2021年第1期76-80,共5页
China Poultry
基金
国家自然科学基金(31560680,31060312)。
