摘要
从湖北省出现肺炎的某猪场育肥猪中分离出一株革兰氏阴性杆菌,经病原分离、革兰氏染色、PCR鉴定、生化鉴定,确定其为溶血性曼氏杆菌(Mannheimia haemolytica)。该菌呈革兰氏阴性的短小杆菌,两极着色。通用引物扩增片段基因序列比对结果显示,该菌基因序列与溶血性曼氏杆菌同源率在99.7%以上,其生化特性符合溶血性曼氏杆菌生化特性。该菌2×10^8CFU/mL可致死小白鼠,具有较强的致病力。药敏试验结果显示,该菌株对氨苄西林、头孢噻肟和环丙沙星等大多数抗菌药物敏感。
A gram negative bacilli was isolated from fattening pigs of a pig farm with pneumonia in Hubei province.After pathogen iso⁃lation,gram staining,PCR identification and biochemical identification,it was identified as Mannheimia haemolytica.The bacterium was gram-negative short bacilli with two poles stained.The results of sequence comparison showed that the homology rate of the gene sequence of the strain was more than 99.7%,and its biochemical characteristics accorded with the biochemical characteristics of the strain.The strain 2×10^8 CFU/mL can kill mice and has strong pathogenicity.The results of drug sensitivity test showed that the strain was sensitive to ampicillin,cefotaxime and ciprofloxacin.
作者
刘泽文
袁芳艳
刘威
高婷
周丹娜
杨克礼
段正赢
郭锐
梁婉
田永祥
LIU Ze-wen;YUAN Fang-yan;LIU Wei;GAO Ting;ZHOU Dan-na;YANG Ke-li;DUAN Zheng-ying;GUO Rui;LIANG Wan;TIAN Yong-xiang(Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Science/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis,Ministry of Agriculture and Aural Affairs,Wuhan 430064,China)
出处
《湖北农业科学》
2020年第24期177-178,188,共3页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目(2016-620-000-001-026)。
