摘要
为实现对染料废水的高效降解,以聚偏氟乙烯(PVDF)分离膜为载体,利用原子转移自由基聚合(ATRP)引发聚甲基丙烯酸缩水甘油酯(PMGA)接枝在膜表面,通过环氧基与伯胺基的反应,制备出固定化漆酶的分离膜,以实现对废水处理截留过程中高效的染料废水降解。结果表明:漆酶固定化后使膜材料具有高效的催化效率,在4 h后均能达到60%以上,最高可至97.2%±1.4%。同时进一步地利用聚乙烯亚胺(PEI)在固定漆酶的PGMA刷间形成交联网络,有益于维持酶构象稳定性从而保留较高的酶活性,而适当的交联浓度能最大程度的提高膜的降解能力,其中0.5 mmol/L PEI交联改性的漆酶固定化膜表现出最佳的降解能力。
In order to realize the high-efficiency degradation of dye wastewater,polyvinylidene fluoride(PVDF)is chosen as the substrate membrane,and first grafted with poly(methacrylic acid)(PGMA)using surface-initiate atom transfer radical polymerization(ATRP)reaction.Subsequently,laccases were covalently bound with the epoxy group of PGMA to prepare the laccase immobilized PVDF-g-PGMA membrane.The results demonstrate that the introduction of laccase on membrane surface enable membrane to degrade dyestuff wastewater effectively.After 4 h of degradation,the degradation rate all got above 60%,and the maximum rate is 97.2%±1.4%.The further crosslinking of polyethyleneimine(PEI)is in favor of maintaining conformational stability and improving the retention of enzymatic activity.Importantly,an appropriate cross-linked concentration can maximize the degradation capacity of membranes,which is verified by the optimal degradation efficiency of the 0.5 mmol/L cross-linked PEI modified laccase immobilized membrane.
作者
何洋
江鹏
赵义平
陈莉
HE Yang;JIANG Peng;ZHAO Yi-ping;CHEN Li(School of Material Science and Engineering,Tiangong University,Tianjin 300387,China;State Key Laboratory of Separation Membranes and Membrane Processes,Tiangong University,Tianjin 300387,China)
出处
《天津工业大学学报》
CAS
北大核心
2020年第6期21-27,共7页
Journal of Tiangong University
基金
国家自然科学基金资助项目(51973158)
天津市自然科学基金资助项目(18JCZDJC99500)。
关键词
聚偏氟乙烯膜
漆酶
原子转移自由基聚合
固定化
染料废水降解
polyvinylidene fluoride(PVDF)membrane
laccase
atom transfer radical polymerization(ATRP)
immobilization
dyestuff wastewater
