摘要
采用荧光光谱、紫外光谱和傅里叶红外光谱法研究不同温度下槲皮素和异槲皮素对猕猴桃糖蛋白GP2的影响。确定了GP2与槲皮素和异槲皮素发生相互作用生成新的复合物,在300 K和310 K温度下的荧光猝灭机制主要是静态猝灭;结合常数分别为107.66 L/moL和63.07 L/moL,对应的结合位点数为0.65和0.57;氢键和范德华力是二者之间主要的相互作用力;通过同步荧光光谱法研究确定了槲皮素和异槲皮素与猕猴桃糖蛋白GP2结合位点更接近色氨酸残基;利用傅里叶红外光谱和紫外光谱研究槲皮素和异槲皮素的加入对糖蛋白GP2构象的变化。
Fluorescence spectroscopy,ultraviolet spectroscopy and Fourier infrared spectroscopy were used to study the effects of quercetin and isoquercetin on kiwi fruit glycoprotein GP2 at different temperatures.It was determined that GP2 interacts with quercetin and isoquercetin to form new complexes.The fluorescence quenching mechanism at 300 K and 310 K was mainly static quenching;the binding constants were 107.66 L/moL and 63.07 L/moL,respectively;the corresponding number of binding sites was 0.65 and 0.57;hydrogen bond and van der Waals force were the main interaction forces between the two complexes.It was determined by synchronous fluorescence spectroscopy that the binding sites of quercetin and isoquercetin and kiwi fruit glycoprotein GP2 were closer to tryptophan residues;Fourier transform infrared spectroscopy(FTIR)and ultraviolet spectroscopy(UV)were used to study the conformation changes of glycoprotein GP2 with the addition of quercetin and isoquercetin.
作者
王岸娜
王振
吴立根
刘晓芳
WANG An-na;WANG Zhen;WU Li-gen;LIU Xiao-fang(School of Food Science,Henan University of Technology,Zhengzhou 450001,Henan,China)
出处
《粮食与油脂》
北大核心
2020年第12期111-116,共6页
Cereals & Oils
基金
国家自然科学基金(31201294)
粮食公益性行业科研专项(201313011)
河南省基础与前沿技术研究计划(152300410077)
河南工业大学省属高校基本科研业务费专项资金(2014YWJC05)。
