摘要
目的:分析羧甲纤维素钠的分子量及其分布,推断其分子构象。方法:采用凝胶渗透色谱与多角度激光光散射(GPC-MALLS)联用技术测定羧甲基纤维素钠的分子量及分子量分布,并根据检测结果推断其分子构象。结果:羧甲纤维素钠样品的分子量在105~106之间;多分散系数PDI值在1.5~4.4之间;分子旋转半径(RMS)与重均分子量之间的关系曲线斜率约为0.2~0.6之间,主要集中在0.3~0.5之间,散点部分呈U型曲线,表明羧甲纤维素钠样品是一个紧密且具有高支化度结构的大分子聚合物。实验发现,同一生产企业不同黏度型号的羧甲纤维素钠样品的分子量与黏度呈正相关性;不同生产企业相同黏度型号的样品其分子量及分子量分布存在较大差异。结论:羧甲纤维素钠的分子量及分子量分布的测定、分子构象的分析,为深入研究羧甲纤维素钠的分子结构奠定了坚实的理论基础。
Objective:To determine the molecular weight and distribution of carboxymethylcellulose sodium and to deduce the molecular conformation.Methods:The molecular weight and molecular weight distribution of carboxymethylcellulose sodium(CMC-Na)were determined by GPC-MALLS,and the molecular conformation was deduced.Results:The results showed that the molecular weight of CMC-Na sample was 105-106;PDI value was 1.5-4.4;the slope of curve between RMS and weight average molecular weight was about 0.2-0.6,mainly between 0.3-0.5,and the scatter part was U-shaped curve,which demonstrated that CMC-Na was a tight and highly branched structure macromolecular polymer.It was found that the molecular weight of CMC-Na samples with different viscosity type was positively correlated with the viscosity in the same manufacturer,and the molecular weight and molecular weight distribution of samples with the same viscosity type in different manufacturers were different significantly.Conclusion:The determination of molecular weight and molecular weight distribution and the analysis of molecular conformation of laid a solid theoretical foundation for the further study on the molecular structure of CMC-Na.
作者
田甜
张阳洋
冉文华
柳艳云
江燕
TIAN Tian;ZHANG Yangyang;RAN Wenhua;LIU Yanyun;JIA Yan(Hubei Institute for Drug Control,Wuhan 430075,China;Hubei Gedian Humanwell Pharmaceutical Co.,Ltd.,Ezhou 436000,China;Hubei Engineering Research Center for Drug Quality Control,Wuhan 430075,China)
出处
《中国药品标准》
CAS
2020年第6期494-498,共5页
Drug Standards of China
基金
国家药典委员会药品标准制修订研究课题(2018Y045)
2019年药品国家评价性抽验任务。
关键词
羧甲纤维素钠
GPC
多角度激光散射
分子量
分子构象
carboxymethylcellulose sodium
GPC
multi-angle laser light scatting
molecular weight
molecular conformation
