摘要
背景:利用京尼平交联β-磷酸三钙和壳聚糖这2种材料,探索一种有效、简便的合成方法,以形成一种形态结构稳定且有良好生物相容性的水凝胶材料,为今后组织工程学应用于骨缺损修复打下基础。目的:探究β-磷酸三钙改性的壳聚糖水凝胶对牙髓干细胞生长与矿化的影响。方法:选取壳聚糖和β-磷酸三钙作为原材料构建不同比例仿生骨组织工程水凝胶,对其进行理化性质检测,包括形貌、孔隙率及浸提液中钙的释放量。然后将人牙髓干细胞与水凝胶共培养,观察牙髓干细胞的生长、黏附与矿化情况。结果与结论:①添加β-磷酸三钙的壳聚糖水凝胶孔隙大小更加均一,形态更加稳定,能够持续释放钙;②添加β-磷酸三钙的水凝胶与牙髓干细胞共培养后显示出了较好的生物相容性,并能够在不添加成骨诱导剂的情况下诱导牙髓干细胞矿化;③实验所得水凝胶具有良好的生物相容性和一定的骨诱导性,为制备新型组织工程学材料提供了新的思路。
BACKGROUND:Using the two materials of genipin crosslinkingβ-tricalcium phosphate and chitosan,we explored an effective and simple synthetic method to form a stable hydrogel material with good biocompatibility.This will lay a foundation for the application of tissue engineering in bone defect repair in the future.OBJECTIVE:To investigate the effects ofβ-tricalcium phosphate modified chitosan hydrogel on the growth and mineralization of dental pulp stem cells.METHODS:Chitosan andβ-tricalcium phosphate were selected as raw materials to construct biomimetic bone tissue engineering hydrogel with different ratios.The physical and chemical properties of the hydrogel were tested,including morphology,porosity and release of calcium in the extract.The human dental pulp stem cells were then co-cultured with hydrogel to observe their growth,adhesion and mineralization.RESULTS AND CONCLUSION:(1)The chitosan hydrogel withβ-tricalcium phosphate was more uniform in pore size and more stable in morphology.The hydrogel could release calcium continuously.(2)The hydrogel added withβ-tricalcium phosphate showed good biocompatibility after co-culture with dental pulp stem cells,and was able to induce mineralization of dental pulp stem cells without adding osteogenic inducer.(3)The hydrogel obtained in this experiment has good biocompatibility and certain osteoinductivity,which provides a new idea for the preparation of new tissue engineering materials.
作者
李鑫平
崔秋菊
曾曙光
冉高英
张兆强
刘显文
方炜
徐帅妹
Li Xinping;Cui Qiuju;Zeng Shuguang;Ran Gaoying;Zhang Zhaoqiang;Liu Xianwen;Fang Wei;Xu Shuaimei(Department of Oral and Maxillofacial Surgery,Stomatological Hospital,Southern Medical University,Guangzhou 510280,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第22期3493-3499,共7页
Chinese Journal of Tissue Engineering Research
基金
广州市科技计划项目(201707010193),项目负责人:曾曙光
国家自然科学基金委青年科学基金项目(81800957),项目负责人:徐帅妹
南方医科大学口腔医院院级项目(PY2019023),项目负责人:崔秋菊。
关键词
牙髓干细胞
Β-磷酸三钙
壳聚糖
水凝胶
成骨
矿化
生物相容性
dental pulp stem cells
β-tricalcium phosphate
chitosan
hydrogel
osteogenesis
mineralization
biocompatibility
