摘要
目的研究可供口腔颌面修复用的全降解多孔镁及镁合金组织工程支架的孔隙特征和生物学性能。方法采用模板复制法,以不同形状的NaCl颗粒为造孔粒子,通过渗流铸造工艺分别获得球形孔结构和不规则多面体孔结构的多孔镁及镁合金组织工程支架,两组支架微结构的主孔尺寸一致,联通孔尺寸分别为200~350μm、50~300μm,孔隙率分别为75%和68%。利用扫描电子显微镜、X射线断层衍射、压缩力学测试、析氢测试、浸泡实验和细胞直接培养测试,比较分析微结构对孔隙特征、力学性能、降解行为和细胞粘附的影响。结果扫描电子显微镜和X射线断层衍射结果显示,球形孔支架具有更好的联通性;微结构中的第二相金属间化合物能显著提高微结构的力学性能;比表面积大、孔隙率低的多孔镁支架析氢速率(降解速率)更快;支架与成骨细胞的共培养结果显示微结构降解过快不利于细胞粘附和存活。结论调控微结构可以改变镁合金组织工程支架联通性、降解速率和力学性能,实现性能可控。
Objective To study the pore characteristics and biological properties of porous magnesium and magnesium alloy scaffolds.Methods Porous magnesium and magnesium alloy with different architectures were obtained by the method of template replication,NaCl particles of different shapes were used as space⁃holder particles.The main pore size of the two groups of scaffolds is the same,the connected pore size is 200-350μm and 50-300μm,and the porosity for the two groups of scaffolds is 75%and 68%,respectively.The effects of micro⁃architectures on pore characteristics,mechanical properties,degradation behavior and cell adhesion were compared and analyzed by scanning electron microscopy(SEM),X⁃ray tomography(micro⁃CT),compressive tests,hydrogen evolution tests,immersion tests and direct cell culture tests.Results SEM and micro⁃CT results showed that the spherical pore structure had better in⁃terconnectivity.The intermetallic compound could significantly improve the mechanical properties of micro⁃architecture.The hydrogen e⁃volution rate of porous magnesium scaffolds with larger specific surface area and lower porosity was faster.The results of scaffold⁃osteo⁃blasts co⁃culture showed that the rapid degradation of micro⁃architectures was not conducive to cell adhesion and survival.Conclusion The interconnectivity,degradation rates and mechanical properties of Mg⁃based scaffolds can be modulated by adjusting the micro⁃ar⁃chitectures achieving the controllable performance.
作者
熊美萍
贾高智
袁广银
XIONG Meiping;JIA Gaozhi;YUAN Guangyin(Department of Stomatology,Fifth People's Hospital Affiliated to Fudan University,Shanghai 200240,China)
出处
《口腔医学》
CAS
2020年第11期971-975,共5页
Stomatology
基金
国家自然科学基金(51571143)
上海市卫生和计划生育委员会科研课题计划(201640281)。
关键词
全降解镁合金骨组织工程支架
孔隙特征
力学性能
降解性能
细胞行为
biodegradable Mg⁃based bone tissue engineering scaffold
pore characteristics
mechanical properties
biodegradation
cell behavior
