摘要
目的研究并建立升降丸的质量标准。方法运用薄层色谱法(TLC)鉴别升降丸中的姜黄和大黄,运用高效液相色谱法测定升降丸中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量。结果TLC图中特征斑点明显,分离度好,阴性对照无干扰。3个批次的升降丸样品中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均含量分别为1.19、0.64、2.77、5.12、0.85 mg/g;分别在6.37~108.90μg/mL(r=0.9997)、6.73~115.00μg/mL(r=0.9994)、6.96~119.00μg/mL(r=0.9997)、6.96~119.00μg/mL(r=0.9999)、3.32~56.80μg/mL(r=0.9998)范围内,与峰面积的积分值呈良好的线性关系;平均回收率分别为89.91%(RSD=2.12%)、92.87%(RSD=1.06%)、91.24%(RSD=1.67%)、93.50%(RSD=2.31%)、88.64%(RSD=2.46%)。结论实验中建立的方法重现性好、操作简便、准确可靠,可应用于升降丸的质量控制。
Objective To research and establish the quality standard of Shengjiang Pills.Methods Thin layer chromatography(TLC)was used to identify Rhizoma Curcumae Longae and Radix et Rhizoma Rhei in Shengjiang Pills.High performance liquid chromatography was used to determine the contents of aloe-emodin,rhein,emodin,chrysophanol,and physcion in Shengjiang Pills.Results The characteristic spots were obvious,the resolution was good,and the negative control had no interference by TLC.The average contents of aloe-emodin,rhein,emodin,chrysophanol,and physcion in three batches of Shengjiang Pills were respectively 1.19,0.64,2.77,5.12,0.85 mg/g.In the range of 6.37-108.90μg/mL(r=0.9997),6.73-115.00μg/mL(r=0.9994),6.96-119.00μg/mL(r=0.9997),6.96-119.00μg/mL(r=0.9999),3.32-56.80μg/mL(r=0.9998),respectively,and had a good linear relationship with the integral value of peak area.The average recovery rates were 89.91%(RSD=2.12%),92.87%(RSD=1.06%),91.24%(RSD=1.67%),93.50%(RSD=2.31%),88.64%(RSD=2.46%).Conclusion The method established in this experiment has good reproducibility,easy to operate,accuracy and reliability,and can be applied to the quality control of Shengjiang Pills.
作者
陆超
奚肇庆
吴磊
田立元
LU Chao;XI Zhaoqing;WU Lei;TIAN Liyuan(Department of Pharmacy,Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Province,Nanjing 210029,China;Department of Emergency,Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Province,Nanjing 210029,China)
出处
《中国医药导报》
CAS
2020年第31期4-7,F0003,共5页
China Medical Herald
基金
国家自然科学基金资助项目(81973589)。
关键词
升降丸
质量标准
薄层色谱法
高效液相色谱法
Shengjiang Pills
Quality standard
Thin layer chromatography
High performance liquid chromatography
