摘要
目的建立非衍生化液相色谱-串联质谱法测定茶叶中草铵膦残留的分析方法。方法茶叶样品经超纯水均质提取,HR-X固相萃取小柱净化,采用非衍生化液相色谱-串联质谱法直接测定,电喷雾离子源负离子模式下采用多反应监测模式监测,同位素内标法定量。结果在优化的条件下,草铵膦在5~200μg/L范围内呈良好的线性关系,相关系数为0.9998,方法定量限为0.1 mg/kg。方法平均回收率为84.2%~104.9%,相对标准偏差低于7.6%。结论本方法前处理简单、准确性好、灵敏度高,可用于绿茶、红茶、乌龙茶、普洱茶等茶叶中草铵膦残留量的测定。
Objective To establish a method for the determination of glufosinate ammonium residue in tea by nonderivative liquid chromatography-tandem mass spectrometry. Methods Tea samples were homogenized and extracted by ultra-pure water and purified by HR-X solid phase extraction column. The contents were directly detected by nonderivatization liquid chromatography-tandem mass spectrometry, monitored by multi-reaction monitoring method under the negative ion mode of electrospray ion source, and quantified by isotopic internal standard method. Results Under optimized conditions, glufosinate-ammonium showed a good linear relationship in the range of 5-200 μg/L, and the correlation coefficient was 0.9998, and limit of quantification was 0.1 mg/kg. The average recoveries were 84.2%-104.9% and the relative standard deviations were less than 7.6%. Conclusion The proposed method is simple, accurate and sensitive, which is suitable for detecting glufosinate-ammonium residue in green tea, black tea, oolong tea and Pu’er tea.
作者
赵佳胤
魏峰
俞秋红
胡贝贞
ZHAO Jia-Yin;WEI Feng;YU Qiu-Hong;HU Bei-Zhen(Nanjing Customs Industrial Products Testing Center,Wuxi,214101,China;Comprehensive Technical Service Center of Shaoxing Customs,Shaoxing,312000,China)
出处
《食品安全质量检测学报》
CAS
2020年第20期7322-7328,共7页
Journal of Food Safety and Quality
关键词
茶叶
草铵膦
非衍生化
液相色谱-串联质谱法
tea
glufosinate-ammonium
non derivatization
liquid chromatography-tandem mass spectrometry
