摘要
浙江省淳安县三叶青叶部病害发生危害严重,对当地三叶青生产造成了巨大的经济损失;明确其病原菌种类与生物学特性,可为病害的有效防控提供理论依据。采用常规组织分离法对病原菌进行分离,利用病原菌形态学、分子系统学(rDNA-ITS、β-tubulin、ACT、gpd、CaM基因的PCR扩增、序列分析和系统发育树的构建)和致病性测定相结合的方法,将为害三叶青的病原菌鉴定为胶孢炭疽菌(Colletotrichum gloeosporioides);并采用十字交叉法和血球计数板计数法对该菌的生物学特性进行了研究。结果表明:菌株CA-2在20~35℃均能生长,最适菌丝生长和产孢的温度均为25℃;24 h暗培养最利于菌丝生长和产孢;培养基pH为5.0最利于菌丝生长,而pH为8.0最利于产孢。菌株CA-2能利用多种碳、氮源营养,但存在一定的差异;其中,菌丝在以麦芽糖和山梨醇为碳源、牛肉浸膏为氮源的培养基上生长最快,在以乙醇为碳源、谷氨酰胺为氮源的培养基上最慢;产孢量在以山梨醇为碳源、酵母膏为氮源的培养基上最大,在以甘露醇为碳源、胰蛋白胨为氮源的培养基上最少。
Leaf spot occurred severely in Chun an city,Zhejiang Province,and caused serious economic losses in local Tetrastigma hemsleyanum production.To clarify the species and biological characteristics of the pathogen caused leaf spot could provide scientific data for the disease effective control.The pathogen was isolated by plate confrontation assay and identified as Colletotrichum gloeosporioides based on combination of morphological characters,molecular data(rDNA-ITS,β-tubulin,ACT,gpd,CaM)and pathogenicity tests.And the biological characteristics determined by crossing and haemocytometer methods showed that the fungus was able to grow at temperature between 20-35℃with an optimum of 25℃for both mycelia growth and sporulation;the continuous darkness was good for both mycelia growth and sporulation;In addition,the optimal pH for mycelia growth and sporulation was 5.0 and 8.0,respectively.There were various kinds of carbohydrates and nitrogen known to provide growth of the fungus.Maltose,sorbitol and yeast were the best carbon and nitrogen source for mycelia growth,respectively,while ethanol and glutamine were the worst,respectively.Sorbitol and yeast were the best carbon and nitrogen source for sporulation,respectively,while mannitol and casein tryptone were the worst,respectively.
作者
李戌清
严建立
阮松林
LI Xuqing;YAN Jianli;RUAN Songlin(Institute of Vegetable,Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China;Branch of Science and Technology,Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China;Institute of Crop(Ecology),Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)
出处
《浙江农业学报》
CSCD
北大核心
2020年第11期2009-2019,共11页
Acta Agriculturae Zhejiangensis
基金
杭州市科委主动设计项目(20190101A05)。
关键词
三叶青
炭疽病
胶孢炭疽菌
病原菌鉴定
生物学特性
Tetrastigma hemsleyanum
anthracnose
Colletotrichum gloeosporioides
identification of pathogen
biological characteristics
