摘要
腺苷在医药和食品领域有广泛的应用。通过发酵工艺调控和培养基优化,提高腺苷发酵得率和糖苷转化率,是工业化生产腺苷的重要途径之一。以枯草芽孢杆菌(Bacillus subtilis)AR090为出发菌株,研究发酵培养基中柠檬酸钠和次黄嘌呤的添加量及添加方式对其生产腺苷的影响。结果表明:发酵培养基中添加2 g/L柠檬酸钠,并在发酵过程的24 h和36 h分2次补充次黄嘌呤(前体)3 g/L,可使腺苷产量大幅度提升,产量最高达到31.1 g/L,相比于原配方工艺腺苷产量(22.3 g/L)提高了39.46%。
Adenosine has a wide range of applications in the fields of medicine and food.Through the adjustment of fermentation process and optimization of the medium,the increase of adenosine fermentation yield and glycoside conversion rate is the requirement of industrialized production of adenosine.A study of fermentation process for adenosine production based on citrate coupling hypoxanthine addition in pulses,with Bacillus subtilis-AR090 as the starting strain.The results of this study show that adding 2 g/L of sodium citrate to the substrate and supplementing 3 g/L of hypoxanthine(precursor)twice during the fermentation for 24 h and 36 h can greatly increase the adenosine production,with the highest yield of 31.1 g/L.Compared with the original formula process,the novel fermentation process for adenosine production(22.3 g/L)increased by 39.46%.
作者
陈华强
CHEN Huaqiang(Star Lake Bioscience Co.,Inc.,Zhaoqing 516100,China)
出处
《发酵科技通讯》
CAS
2020年第4期211-214,共4页
Bulletin of Fermentation Science and Technology
