摘要
运用超高效液相色谱建立粮食中L-色氨酸含量的检测方法。样品经过6 mol/L氢氧化钠和5%氯化亚锡配成的水解保护液,在110℃烘箱下水解20 h,经Dikmatech C18色谱柱(Endeavorsil 1.8μm 50 mm×2.1 mm),磷酸氢二钠缓冲液(pH3.0)为流动相等梯度洗脱,DAD检测器278 nm,在0.728~14.56μg/mL范围内线性良好。标准曲线方程为Y=9.531X—1.0395,相关系数R2=0.9997,L-色氨酸最低检出限为0.006%,最低定量限为0.019%。粮食中如稻谷和玉米,L-色氨酸含量小于0.1%,该方法精密度≤10%;粮食中如小麦和大豆,L-色氨酸含量高于0.1%时,该方法精密度≤3%,加标回收率在90.07%~96.27%。该方法检测时间短,准确可靠,适用于粮食中色氨酸的定量检测。
A method for the determination of L-tryptophan in grain was established by ultra high performance liquid chromatography.Samples after 6 mol/L NaOH and 5%match hydrolysis protection of SnCl2 hydrolysis of 20 h under 110℃oven,via Dikmatech C18 chromatographic column(Endeavorsil 1.8μm 50 mm×2.1 mm),Na2HPO4 buffer(pH3.0)as equal flow gradient elution,DAD detector,278 nm in 0.728~14.56μg/mL range linear good.Standard curve equation for Y=9.531X—1.0395,R2=0.9997,the minimum detection limit of L-tryptophan was 0.006%,and the minimum quantitative limit was 0.019%.When the content of L-tryptophan in grains such as rice and maize is less than 0.1%,the precision of this method is less than or equal to 10%;when the content of L-tryptophan in grains such as wheat and soybean is higher than 0.1%,the precision of this method is less than or equal to 3%,and the standard recovery rate is between 90.07%and 96.27%.The method is accurate and reliable,and is suitable for the quantitative determination of tryptophan in grain.
作者
单晓雪
SHAN Xiaoxue(Sinograin Chengdu Storage Research Institute Co.,Ltd.,Chengdu 610000,China)
出处
《食品科技》
CAS
北大核心
2020年第9期281-286,共6页
Food Science and Technology
基金
2018~2020年度中国科协青年人才托举工程项目(2018QNRC001)。
关键词
粮食
L-色氨酸
超高效液相色谱
碱水解
grain
L-tryptophan
ultra high performance liquid chromatography
alkali hydrolysis
