摘要
目的探究蒌芩止嗽优化方对机体铜绿假单胞菌(PA)感染的免疫保护作用。方法体外实验观察蒌芩止嗽优化方对左氧氟沙星透过PA成熟生物被膜(BF)的影响。体内实验观察其对PA感染大鼠肺部组织TNF-α、IFN-γ的影响,探究蒌芩止嗽优化方对炎症机体免疫失衡的调控。结果体外,蒌芩止嗽优化方与氨溴索均可以协助左氧氟沙星透过PA成熟生物被膜。体内,西药组与中药组均可促进肺部细菌的清除,并且有助于减轻肺组织的病理损伤;中药组与模型组比较肺部的TNF-α水平明显降低(P<0.001),IFN-γ水平显著提高(P<0.001);西药组与模型组比较肺部的TNF-α水平明显降低(P<0.001),IFN-γ水平虽轻微提高,但差异无统计学意义;中药组与西药组比较肺部的TNF-α水平明显降低(P<0.001),IFN-γ水平显著提高(P<0.001)。结论蒌芩止嗽优化方体外可协助抗生素透过成熟生物被膜,其机制可能是通过纠正PA感染大鼠体内炎症免疫失衡,一方面加快了肺部细菌的清除及减轻肺部病理损伤,从而减轻机体炎症反应;另一方面增强机体对PA生物被膜感染的抵抗力,从而间接达到清除细菌的作用,它与氨溴索比较的优势可能亦在于可以提高机体免疫力,其深层作用机制尚有待进一步深入探讨。
Objective To explore the immune protective effects of Louqin Zhisou Youhua Fang(Snakegourd-fruit Scutellaria-root Cough-stopping Formula with Modifications, LQZSYHF) on infection with Pseudomonas aeruginosa. Methods The effects of LQZSYHF on improving the penetration of levofloxacin through the PA mature biofilm(BF) were studied via in vitro experiment. Its effects on regulating inflammatory immune imbalance were explored through observing the levels of TNF-α and IFN-γ in the lung tissues of PA-infected rats via in vivo experiment. Results In vitro, both LQZSYHF and ambroxol assisted levofloxacin in penetrating PA mature biofilms. In vivo, both the Western drug and Chinese herbal medicine significantly promoted the elimination of lung bacteria and reduced the pathological damage to lung tissue. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the Chinese herbal medicine(CHM) group compared with the model group(P<0.001). TNF-α level in lung tissue was greatly significantly reduced(P<0.001) and IFN-γ level was slightly increased without statistical significance in the Western drug(WG) group compared with the model group. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the CHM group compared with WD group(P <0.001). Conclusion LQZSYHF can help antibiotics pass through mature biofilms in vitro. Its mechanism might be that it can correct the immune imbalance in PA-infected rats, by which it accelerates the elimination of lung bacteria and reduces lung pathological damage, thereby reducing inflammatory response, and it enhances body resistance against PA biofilm infection, thereby indirectly eliminating bacteria. Compared with ambroxol, its advantages may lie in improving immunity, and its underlying mechanism warrants further study.
作者
李杰
吴童
于子洋
刘丽婷
黄明明
赵翠云
孟子霞
彭桂英
Li Jie;Wu Tong;Yu Ziyang;Liu Liting;Huang Mingming;Zhao Cuiyun;Meng Zixia;Peng Guiying(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700 China;School of Life Sciences,Beijing Universih of Chinese Medicine,Beijing 102488,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2020年第9期754-761,共8页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金青年基金项目(No.81202681)
北京中医药大学2019年度基本科研业务费创新科研团队项目(No.2019-JYB-TD014)。
关键词
蒌芩止嗽优化方
炎症免疫失衡
抗生素
铜绿假单胞菌
生物被膜
大鼠
Louqin Zhisou Youhua Fang
inflammatory immune imbalance
antibiotic
Pseudomonas aeruginosa
biofilm
rats
