摘要
目的探讨表观遗传抑制因子PcG家族成员果蝇zeste基因增强子的人类同源物1/2(EZH1/EZH2)、胚胎外胚层发育蛋白(EED)及胚胎干细胞抑制蛋白(SUZ12)在常见皮肤T细胞淋巴瘤及淋巴组织增殖性疾病(CTCL/LPD)中的表达。方法收集2012—2019年于中国医学科学院皮肤病医院确诊的93例CTCL/LPD及8例扁平苔藓皮损石蜡标本,行免疫组化染色,观察EZH2、EED、SUZ12及EZH1蛋白表达。采用SPSS 25.0软件进行卡方检验及Spearman相关分析。结果93例中包括44例蕈样肉芽肿(MF)、17例NK/T细胞淋巴瘤(NK/TCL)及原发性皮肤间变大细胞淋巴瘤(PC-ALCL)、淋巴瘤样丘疹病(LyP)、种痘水疱病样淋巴组织增殖性疾病(HV-like LPD)及皮下脂膜炎样T细胞淋巴瘤(SPTCL)各8例。93例CTCL/LPD中83例(89.2%)EZH2、81例(87.1%)EED、78例(83.9%)SUZ12、37例(39.8%)EZH1阳性;8例扁平苔藓中1例EZH2、8例EZH1阳性,EED、SUZ12全阴性。CTCL/LPD与扁平苔藓4种蛋白的表达分级差异均有统计学意义(χ2分别为41.75、39.74、39.36及32.83,均P<0.001),且MF、NK/TCL、PC-ALCL、LyP、HV-like LPD及SPTCL与扁平苔藓的表达差异亦均有统计学意义(α=0.0083,均P<0.001)。同时,EZH2与EZH1的表达评分在MF、NK/TCL、PC-ALCL、LyP、HV-like LPD及SPTCL中均呈负相关(rs分别为-0.60、-0.68、-0.89、-0.74、-0.93、-0.80,均P<0.05)。结论PcG家族成员EZH2、EED、SUZ12及EZH1在CTCL/LPD中表达异常。
Objective To investigate the expression of epigenetic inhibitor polycomb group proteins such as enhancer of zeste homolog 1/2(EZH1/EZH2),embryonic ectoderm development protein(EED)and suppressor of zeste 12(SUZ12)in common cutaneous T-cell lymphomas and lymphoproliferative disorders(CTCL/LPD).Methods Totally,93 paraffin-embedded skin samples of CTCL/LPD and 8 of lichen planus were collected from Hospital for Skin Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College between 2012 and 2019,and subjected to immunohistochemical staining to determine the protein expression of EZH2,EED,SUZ12 and EZH1.Statistical analysis was carried out with SPSS 25.0 software by using chi-square test and Spearman correlation analysis.Results The 93 cases of CTCL/LPD included 44 cases of mycosis fungoides(MF),17 natural killer/T cell lymphoma(NK/TCL),8 primary cutaneous anaplastic large cell lymphoma(PC-ALCL),8 lymphomatoid papulosis(LyP),8 hydroa vacciniforme-like lymphoproliferative disorder(HV-like LPD)and 8 cases of subcutaneous panniculitis-like T cell lymphoma(SPTCL).Among the 93 CTCL/LPD cases,83(89.2%)were positive for EZH2,81(87.1%)for EED,78(83.9%)for SUZ12 and 37(39.8%)for EZH1;among the 8 cases of lichen planus,1 was positive for EZH2,all were positive for EZH1,and all were negative for EED and SUZ12.The expression of EZH2,EED,SUZ12 and EZH1 in lichen planus samples significantly differed from all the CTCL/LPD samples(χ2=41.75,39.74,39.36,32.83,respectively,all P<0.001),and from MF,NK/TCL,PC-ALCL,LyP,HV-like LPD and SPTCL samples separately(α=0.0083,all P<0.001).Meanwhile,the score of EZH2 expression was negatively correlated with that of EZH1 expression in the MF,NK/TCL,PC-ALCL,LyP,HV-like LPD and SPTCL tissues(rs=-0.60,-0.68,-0.89,-0.74,-0.93,-0.80,respectively,all P<0.05).Conclusion Polycomb group proteins EZH2,EED,SUZ12 and EZH1 are abnormally expressed in CTCL/LPD lesions.
作者
甘璐
张莹
石浩泽
宋昊
王雅洁
程伟
邵雪宝
英成双
陈浩
孙建方
Gan Lu;Zhang Ying;Shi Haoze;Song Hao;Wang Yajie;Cheng Wei;Shao Xuebao;Ying Chengshuang;Chen Hao;Sun Jianfang(Department of Pathology,Hospital for Skin Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College,Nanjing 210042,China)
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2020年第11期880-885,共6页
Chinese Journal of Dermatology
基金
江苏省六大人才高峰项目(WSN-030)
中国医学科学院医学与健康科技创新工程项目(CIFMS-2017-I2M-1-017)
北京协和医学院研究生创新基金(3301030202026)。
