摘要
旨在探究睡眠剥夺后小鼠血浆褪黑激素(MT)水平和下丘脑钟基因表达的变化。将72只小鼠随机分为对照组与睡眠剥夺试验组(n=36),试验组小鼠接受水平台法连续72 h睡眠剥夺。在试验结束后当日每隔4 h(CT4、8、12、16、20和24时)采血测定血浆MT含量,并取下丘脑以RT-PCR方法测定钟基因mClock、mBmal1、mCry1/2、mPer1~3、mRorβ和mReverbα表达量。结果显示,相较于对照组,试验组小鼠血浆MT水平昼夜节律紊乱。在下丘脑钟基因的表达量上,mClock、mRorβ和mReverbα的中值显著下降(P<0.05),而mPer1和mPer2中值显著增加(P<0.01);对于昼夜节律,mCry1和mCry2的昼夜节律消失;对于钟基因的相位,mBmal1和mClock相位提前,而mRorβ和mPer1~3相位延后。结果提示,小鼠睡眠剥夺3 d可致MT分泌和下丘脑钟基因表达节律紊乱。
This study was conducted to investigate the changes in plasma Melatonin(MT)level and hypothalamic clock gene expression in mice after sleep deprivation(SD).Seventy-two mice were randomly divided into SD group and non-sleep-deprived control group(n=36).The SD mice were subjected to a modified multiple platform water path for 72 h sleep deprivation.After that,blood samples were taken every four hours(CT4,8,12,16,20 and 24)for the detection of plasma MT content,and hypothalamus samples were collected for the detection of the expressions of clock genes including mClock,mBmal 1,mCry 1/2,mPer 1-3,mRorβand mReverbα.The results showed that compared with control group,circadian rhythm of plasma MT was disordered in SD group.The median values of mClock,mRorβand mReverbαin the hypothalamus decreased significantly(P<0.05),but mPer1 and mPer2 increased significantly(P<0.01);the circadian rhythms of mCry 1 and mCry 2 disappeared;the expression rhythms of mBmal 1,and mClock were advanced in phase,while mRorβand mPer 1-3’s were delayed.These results suggested that acute sleep deprivation of 3 d could cause disorders of MT secretion and hypothalamic clock gene expression in mice.
作者
杨悦
郭泽明
韩昊书
李伟天
高婷
王心彤
陈耀星
YANG Yue;GUO Zeming;HAN Haoshu;LI Weitian;GAO Ting;WANG Xintong;CHEN Yaoxing(Laboratory of Anatomy of Domestic Animals,College of Animal Medicine,China Agricultural University,Beijing 100193,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2020年第10期2609-2612,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家级大学生创新计划(201810019098)
北京市自然科学基金(6182018)。
关键词
睡眠剥夺
褪黑激素
钟基因
下丘脑
昼夜节律
sleep deprivation
melatonin
clock gene
hypothalamus
circadian rhythm
