摘要
利用响应面法优化浙贝母蒽醌的超声提取工艺,并评价其抑菌活性。在超声功率为100 W条件下,以浙贝母蒽醌提取率为响应值,考察料液比、提取时间和乙醇浓度等单因素对其提取率的影响。经实验研究优化后得到的提取工艺为:料液比1∶22 g/mL、提取时间22 min、乙醇浓度74%。此时,浙贝母蒽醌的提取率为0.1587%,与预测值相差极小,说明该工艺可行,可用于浙贝母蒽醌的提取。同时采用牛津杯法抑菌试验发现,浙贝母蒽醌对大肠杆菌、金黄色葡萄球菌均有一定的抑菌作用,且随着浙贝母蒽醌浓度的增加,抑菌圈直径呈增大趋势;当浙贝母蒽醌溶液浓度为10 mg/mL时,对这两种菌的抑制效果最好,且对金色葡萄球菌的抑菌效果强于大肠杆菌。
Response Surface Methodology(RSM)was used to optimize the ultrasonic extraction process of anthraquinone from Fritillaria thunbergii and to evaluate its antibacterial activity.Under the condition of 100 W ultrasonic power,the extraction rate of total anthraquinone was taken as the response value,and the effects of single factors,such as material ratio,extraction time,and ethanol concentration on the extraction rate of total anthraquinone in shellfish were investigated.After the optimization,the extraction process of the total beryllium was as follows:the sold-liquid ratio was 1∶22 g/mL,the extraction time was 22 min,and the ethanol concentration was 74%.Under this condition,the extraction rate of total anthraquinone in shellfish was 0.1587%,which was little different from the prediction value.It showed that the process was feasible and could be used for the extraction of anthraquinone from Fritillaria thunbergii.At the same time,the Oxford Cup bacteriostatic test showed that the total anthraquinone of Fritillaria thunbergii had certain bacteriostatic effect on Escherichia coli and Staphylococcus aureus,and the diameter of bacteriostatic zone increased with the increase of the anthraquinone concentration.When the concentration of anthraquinone solution was 10 mg/mL,the inhibitory effect on both bacteria was the best,and the inhibitory effect on Staphylococcus aureus was stronger than that on E.coli.
作者
侯敏娜
侯少平
刘艳红
王珊
许海燕
HOU Min-na;HOU Shao-ping;LIU Yan-hong;WANG Shan;XU Hai-yan(Shaanxi International Business College,Xianyang 712046,China)
出处
《江西农业学报》
CAS
2020年第10期81-86,共6页
Acta Agriculturae Jiangxi
基金
陕西省教育厅项目(18JK0955)
陕西国际商贸学院中药质量标志物创新团队项目(SSY18TD02)
陕西省大学生创新创业训练计划项目(201849006)
陕西省科研平台项目(2019XT-2-202)。
关键词
浙贝母
蒽醌
超声提取
响应面法
抑菌活性
Fritillaria thunbergii
Quinone
Ultrasonic extraction
Response surface methodology
Antibacterial activity
