摘要
目的:探讨舌状鳞癌组织中Krüxxppel样转录因子(KLF6)和G-蛋白偶联受体124(GPCR124)表达水平及其与临床病理特征和预后的相关性。方法:64例手术治疗的舌状鳞癌患者,术前收集患者血液样本并于术中获取舌状鳞癌组织样本,采用免疫组织化学方法检测KLF6和GPCR124的表达水平。对所有患者性别、年龄、临床分期、组织分化程度、预后评分、KLF6和GPCR124蛋白表达情况及其预后情况进行记录,并比较KLF6和GPCR124不同表达患者间上述资料间的差异。患者出院后均随访5年。结果:KLF6和GPCR124蛋白在舌状鳞癌组织中分别呈低表达和高表达,即舌状鳞癌组织中KLF6蛋白阳性率表达低于正常组织,而GPCR124蛋白高于正常组织(P<0.05)。年龄、性别与舌状鳞癌患者KLF6和GPCR124蛋白表达无关(P>0.05);但临床分期和组织分化程度与舌状鳞癌患者KLF6和GPCR124蛋白表达有关(P<0.05)。GPCR124蛋白高表达和KLF6蛋白低表达的患者5年总生存率均分别低于其低表达和高表达患者(χ^2=4.232,P=0.014;χ^2=9.218,P=0.002)。分析得出不同临床分期、组织分化程度、预后评分、KLF6和GPCR124蛋白表达舌状鳞癌患者的5年生存率比较,差异有统计学意义(P<0.05);舌状鳞癌患者预后的独立危险因素为临床Ⅲ~Ⅳ期、低分化组织、预后评分超过7分及KLF6蛋白低表达和GPCR124高表达(P<0.05)。结论:GPCR124高表达和KLF6低表达与患者的肿瘤临床病理特征及预后有关,呈高表达状态的KLF6和低表达状态的GPCR124联合预后评分超过7分、临床Ⅲ~Ⅳ期和低分化组织影响舌状鳞癌患者预后,GPCR124与KLF6蛋白有可能作为理想的舌状鳞癌的预后标志物。
Objective:To investigate the expression levels of Krüxxppel-like factor 6(KLF6)and G pro-tein-coupled receptor 124(GPCR124)in tissues of ligulate squamous cell carcinoma,and to investigate the correlation with clinicopathological characteristics and prognosis.Methods:64 patients with ligulate squamous cell carcinoma were selected.Blood samples of patients were collected before surgery and tissue samples of ligulate squamous cell carcinoma were obtained during the operation.The expression levels of KLF6 and GPCR124 were detected by immunohistochemistry.All patients gender,age,clinical stage,degree of tissue differentiation,prognostic score,KLF6 and GPCR124 protein expression and their prognosis were recorded,and the differences in the above data among patients with different expressions of KLF6 and GPCR124 were compared.All patients were followed up for 5 years after treatment.Results:The expression of KLF6 and GPCR124 proteins in ligulate squamous cell carcinoma tissues was low and high,respectively.That is,the expression of KLF6 protein in ligulate squamous cell carcinoma tissues was significantly lower than that in normal tissues,while GPCR124 protein was significantly higher than that in normal tissues(P<0.05).The age and gender were not related to KLF6 and GPCR124 protein expressions in patients with ligulate squamous cell carcinoma(P>0.05).However,the clinical stage and the degree of tissue differentiation were related to the expressions of KLF6 and GPCR124 proteins in patients with ligulate squamous cell carcinoma(P<0.05).The 5-year overall survival rates of patients with high expression of GPCR124 protein and low expression of KLF6 protein were significantly lower than those with low expression and high expression,respectively(χ2=4.232,P=0.014 vs.χ2=9.218,P=0.002).Analysis showed that there were differences in 5-year survival rates in patients with different clinical stages,the degree of tissue differentiation,prognostic scores,and KLF6 and GPCR124 protein expressions in ligulate squamous cell carcinoma(P<0.05).The independent risk factors affecting the prognosis of patients with ligulate squamous cell carcinoma were clinical stagesⅢtoⅣ,poorly differentiated tissues,prognostic scores>7 points,low expression of KLF6 protein and high expression of GPCR124(P<0.05).Conclusion:The high expression of GPCR124 and the low expression of KLF6 are related to the clinicopathological characteristics and prognosis of patients.The high KLF6 level with low GPCR124 level combined with prognostic score>7 points,clinical stagesⅢtoⅣand poorly differentiated tissues affect the prognosis of patients with ligulate squamous cell carcinoma.GPCR124 and KLF6 proteins may be ideal prognostic markers of ligulate squamous cell carcinoma.
作者
徐志健
杨晓倩
吕建建
XU Zhi-jian;YANG Xiao-qian;LV Jian-jian(Department of Oncology,Qingdao Municipal Hospital,Qingdao 266011,Shandong,China)
出处
《川北医学院学报》
CAS
2020年第5期790-793,共4页
Journal of North Sichuan Medical College
基金
山东省卫计委科研课题(18J200213)。
