摘要
目的:本课题拟评价孟鲁司特钠治疗变异性哮喘模型小鼠的效果。方法:采用卵清蛋白(ovalbumin,OVA)经致敏、激发来复制哮喘模型并予以药物治疗。首先对实验动物进行分组,分为空白组、模型组、孟鲁司特钠组,每组各6只小鼠,然后进行雾化建模及灌胃和腹腔注射给药。结果:孟鲁司特钠组小鼠血清中IL-2含量增高,孟鲁司特钠组小鼠血清中TNF-α含量降低;孟鲁司特钠组与模型组相比较,小鼠肺湿重有明显减少,存在显著差异(P<0.05),且小鼠肺干重及肺湿干重比值均降低了;孟鲁司特钠组小鼠白细胞总数、中性粒细胞及嗜酸性粒细胞、单核细胞、淋巴细胞均明显减少。结论:孟鲁司特钠对改善小鼠咳嗽变异性哮喘(cough variant asthma,CVA)症状具有良好疗效。
Objective:This study is to evaluate the efficacy of montelukast sodium in the treatment of variant asthma mice.Method:The ovalbumin(OVA)is sensitized and stimulated to replicate the asthma model and be treated with drugs.The experimental animals were first divided into blank group,model group and montelukast sodium group,each group of 6 mice,then subjected to atomization modeling and intragastric and intraperitoneal injection to feed the mice.Results:The serum il-2 content in the montelukast sodium group was increased,while the TNF-alpha content in the montelukast sodium group was decreased.Compared with the model group,the lung wet weight of mice in the montelukast sodium group was significantly reduced,with a significant difference(P<0.05),and the lung dry weight and lung wet dry weight ratio of mice were reduced.The total number of white blood cells,neutrophils,eosinophils,monocytes and lymphocytes in montelukast sodium group were significantly decreased.Conclusion:The results showed that montelukast sodium has a good effect on improving the symptoms of cough variant asthma(CVA)in mice.
作者
许慧琳
王昊
王福玲
刘鑫阳
文豆玉
王隆权
Xu Huilin(School of Pharmacy,Harbin University of Commerce,Harbin Heilongjiang 150076)
出处
《黑龙江医药》
CAS
2020年第5期984-987,共4页
Heilongjiang Medicine journal
基金
国家级大学生创新创业训练计划项目(编号:201810240049)。
