摘要
为解决在放置一段时间后海蜇(Rhopilema esculentum)毒素溶血活性锐减的问题,利用分光光度计测量了EDTA、GSH、Ca^2+、NaCl等化学因素对海蜇毒素溶血活性的稳定效果。结果发现:(1)EDTA对毒素具有较好的稳定作用,浓度在0.5 mmol/L时溶血活性最高,其溶血率为93.63%;(2)NaCl对毒素也具有稳定作用,当浓度在5 mmol/L时溶血活性最高,其溶血率为92.68%;(3)GSH对毒素稳定作用较差,在浓度为0.5 mmol/L时溶血活性最高,其溶血率为58.17%;(4)Ca^2+对海蜇毒素不具有稳定作用;(5)正交试验发现,NaCl对溶血活性的稳定性影响最大,在GSH、NaCl、EDTA浓度分别为1.0、5、0 mmol/L时,海蜇毒素的溶血性最强。
In order to solve the problem that the hemolytic activity of jellyfish(Rhopilema esculentum)toxin decreases sharply after being placed for a period of time,the stabilizing effect of chemical factors such as EDTA,GSH,Ca^2+and NaCl on the hemolytic activity of jellyfish toxin was measured by spectrophotometer.The results indicated that:(1)The presence of EDTA benefited the stability of the hemolytic activity.The hemolytic activity was the highest when the concentration was 0.5 mmol/L,and its hemolysis rate was 93.63%.(2)NaCl also had a stabilizing effect on the toxin.When the concentration was 5 mmol/L,the hemolytic activity was the highest and the hemolysis rate was 92.68%.(3)GSH had a poor stabilizing effect on the toxin.The hemolytic activity was the highest when the concentration was 0.5 mmol/L,and its hemolysis rate was 58.17%.(4)The results showed that Ca^2+had no stabilizing effect on jellyfish toxin.(5)Orthogonal experiment found that NaCl had the greatest influence on the stability of hemolytic activity.When the concentrations of GSH,NaCl,and EDTA were 1.0,5,and 0 mmol/L,respectively,the hemolysis of jellyfish toxin is the strongest.
作者
李伟
李红
周宇
徐善良
LI Wei;LI Hong;ZHOU Yu;XU Shan liang(School of Marine Sciences,Ningbo University,Ningbo 315211,China;Key Laboratory of Applied Marine Biotechnology Ministry of Education,Ningbo University,Ningbo 315211,China)
出处
《河北渔业》
2020年第10期9-13,23,共6页
基金
浙江省重大科技专项项目(2019C02059)。
