摘要
目的探讨粪菌移植(fecal microbiota transplantation,FMT)对非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠肠黏膜屏障的保护作用。方法健康雄性SD大鼠30只,随机分为3组:正常对照组(control group,C组)10只,予正常饮食;高脂模型组(model group,M组)10只、粪菌移植治疗组(treatment group,T组)10只,M组和T组均予高脂饮食。T组予粪菌液灌胃2 mL/次,隔日1次,粪菌液灌胃的前一天晚上及当天早上均予奥美拉唑镁肠溶片灌胃;C组及M组同时予奥美拉唑及生理盐水灌胃。喂养12周后实验结束,测定血中TG、ALT、AST水平;苏丹黑B染色观察肝脏病理学变化;取回肠末端肠组织行HE染色及扫描电镜观察肠黏膜结构变化。结果与M组大鼠相比,T组血清TG、ALT、AST水平降低,差异有统计学意义(均P<0.05)。T组大鼠肝脏苏丹黑B染色可见肝细胞内脂肪沉积明显减少,脂肪变性程度较M组减轻。T组大鼠肠组织HE染色肠绒毛轻度水肿,排列较整齐、紧密。扫描电镜中可见T组大鼠肠绒毛形态较饱满,排列比较紧密,微绒毛之间的间隙变小。结论粪菌移植能改善肝功能,减轻肝脏脂肪变,降低肠道通透性,改善肠黏膜屏障功能。
Objective To explore the protective effects of fecal microbiota transplantation(FMT)on intestinal mucosal barrier of nonalcoholic fatty liver disease(NAFLD)rats.Methods Thirty healthy male Sprague-Dawley rats(weighted 180 g±20 g)were randomized into three groups,the control group(group C),the model group(group M)or the treatment group(group T),10 rats in each group.Normal diets were given to group C.Rats in group M and group T were given high-fat diets,then the rats in group T were given 2 mL fecal bacteria liquid through intragastric administration every other day;In the night before FMT and the morning of the day,the rats were given omeprazole magnesium enteric-coated tablets in a dose of 10 mg/kg by gavage.Group C and group M were given the same amount of normal saline.12 weeks later,2 rats in each group were randomly killed to confirm successful modeling using Sultan black B staining.The levels of serum TG,ALT and AST were detected.The pathological changes of liver were observed by using Sudan Black B staining.The changes of intestinal mucosa structure were observed using HE staining and scanning electron microscope.Results Compared with group M,the levels of TG,ALT and AST in group T were significantly higher(all P<0.05).After receiving FMT,the fat deposition in the liver cells in group T was significantly reduced,and the degree of steatosis was reduced,compared with the group M.HE staining showed mild edema in intestinal tissue of rats in group T,with the intestinal villi arranged more neatly.Scanning electron microscopy showed that,compared with group M,the intestinal villi were plump and arranged tightly,with smaller gaps between them.Conclusion FMT can reduce hepatic steatosis and improve the liver function,reduce the permeability of intestine and impairment of intestinal mucosal barrier function in NAFLD rats.
作者
李月芹
赵红伟
郑吉敏
王玉珍
LI Yueqin;ZHAO Hongwei;ZHENG Jimin;WANG Yuzhen(Department of Gastroenterology,Hebei General Hospital,Shijiazhuang,Hebei 050051,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2020年第8期893-896,共4页
Chinese Journal of Microecology
关键词
粪菌移植
非酒精性脂肪肝
动物模型
肠黏膜屏障
Fecal microbiota transplantation
Nonalcoholic fatty liver disease
Experimental animal
Rectum mucosal barrier
