摘要
目的:建立HPLC法同时测定济川合剂中咖啡酸、松果菊苷、阿魏酸、异阿魏酸、毛蕊花糖苷、柚皮苷、新橙皮苷共7个成分含量。方法:采用Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,检测波长330 nm,体积流量1.0 mL·min^-1,柱温30℃。结果:7个成分分离度均良好,阴性无干扰;咖啡酸、松果菊苷、阿魏酸、异阿魏酸、毛蕊花糖苷、柚皮苷、新橙皮苷的质量浓度分别在5.070~0.202 8μg·mL^-1(r=0.999 8)、129.9~5.195μg·mL^-1(r=0.999 9)、27.38~1.095μg·m L^-1(r=0.999 4)、19.19~0.767 4μg·m L^-1(r=0.999 3)、77.37~3.095μg·m L^-1(r=0.999 9)、303.2~12.13μg·m L^-1(r=0.999 6)、314.7~12.59μg·m L^-1(r=0.999 7)范围内与峰面积呈良好的线性关系;平均回收率(n=6)依次为102.7%(RSD=1.6%)、98.1%(RSD=1.3%)、100.8%(RSD=2.8%)、97.7%(RSD=2.1%)、99.3%(RSD=1.9%)、101.2%(RSD=2.3%)、101.8%(RSD=1.7%)。3批济川合剂中上述7个成分含量依次为0.025 6~0.025 9、0.492 7~0.494 8、0.099 5~0.099 7、0.076 5~0.076 8、0.219 5~0.230 8、1.271 2~1.275 3、1.285 4~1.289 0 mg·m L^-1。结论:本方法建立的检测方法简便可靠,重现性好,专属性强,可用于济川合剂的质量控制和评价。
Objective:To establish an HPLC method for simultaneous determination of 7 active components(caffeic acid,echinacea glycoside,ferulic acid,isoferulic acid,mullein glycoside,naringin and neohesperidin) in Jichuan mixture. Methods:The analysis was performed on an Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm) with mobile phase consisting of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution at a flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃ and the detection wavelength was 330 nm. Results:Separation of 7 components was satisfactory without interference. The linear ranges of caffeic acid,echinacea glycoside,ferulic acid,isoferulic acid,mullein glycoside,naringin and neohesperidin were 5.070-0.202 8 μg·mL^-1(r=0.999 8),129.9-5.195 μg·mL^-1(r=0.999 9),27.38-1.095 μg·mL^-1(r=0.999 4),19.19-0.767 4 μg·mL^-1(r=0.999 3),77.37-3.095 μg·mL^-1(r=0.999 9),303.2-12.13 μg·mL^-1(r=0.999 6),314.7-12.59 μg·mL^-1(r=0.999 7),respectively. The average recoveries(n=6) were 102.7%(RSD=1.6%),98.1%(RSD=1.3%),100.8%(RSD=2.8%),97.7%(RSD=2.1%),99.3%(RSD=1.9%),101.2%(RSD=2.3%)and 101.8%(RSD=1.7%),respectively. The contents of the above 7 components in 3 batches of samples were 0.025 6-0.025 9,0.492 7-0.494 8,0.099 5-0.099 7,0.076 5-0.076 8,0.219 5-0.230 8,1.271 2-1.275 3 and 1.285 4-1.289 0 mg·mL^-1,respectively. Conclusion:This method is simple,reproducible,accurate and can be used for the quality control of Jichuan mixture.
作者
冯建安
施崇精
李希
黄嫣
王玉
陈娇娇
李双双
FENG Jian-an;SHI Chong-jing;LI Xi;HUANG Yan;WANG Yu;CHEN Jiao-jiao;LI Shuang-shuang(Institute of Traditional Chinese Medicine,Sichuan Academy of Chinese Medical Sciences,Chengdu 610031,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2020年第8期1349-1354,共6页
Chinese Journal of Pharmaceutical Analysis
基金
四川省公益性科研院所基本科研项目(2018-4-502)
中央引导地方科技发展专项项目(2018TZYD0003)。
关键词
济川合剂
高效液相色谱法
咖啡酸
松果菊苷
阿魏酸
异阿魏酸
毛蕊花糖苷
柚皮苷
新橙皮苷
药效成分
Jichuan mixture
HPLC method
caffeic acid
echinacea glycoside
ferulic acid
isoferulic acid
mullein glycoside
naringin
neohesperidin
effective components
