摘要
芸薹根肿菌侵染引起的根肿病是影响甘蓝类蔬菜产量和品质的主要病害之一,抗病品种的培育是防治根肿病最经济有效的方法。本研究以根肿菌4号生理小种为菌源,对花椰菜、结球甘蓝、青花菜、芥蓝和苤蓝6个甘蓝变种共306份品种(系)进行苗期根肿病抗性鉴定,结果显示,甘蓝类抗根肿病材料缺乏,仅在青花菜中发现1份高抗(HR)材料,感病(S)和高感(HS)材料比例占65%以上,中抗(MR)材料在松花菜和结球甘蓝中所占比例均在30%以上。对抗病比例和平均病情指数2个指标的联合评价结果显示,结球甘蓝整体抗性水平高于其他变种,芥蓝和苤蓝抗性最差。另外,对结球甘蓝‘先甘336’(R)×甘蓝/青花菜杂交后代及花椰菜‘托尼’(MR)×青花菜的杂交后代进行苗期根肿病抗性鉴定,结果表明,杂交后代相对于父本病情指数都有所降低,抗性相对提高,且父本病情指数越低,后代表现较高抗性的概率越大。鉴于此,在今后抗病组合的选择与选配过程中,父本和母本最好都具有较高的抗性。本研究将为甘蓝类蔬菜抗根肿病品种培育提供材料支撑和技术参考。
Clubroot,caused by Plasmodiophora brassicae,has been one of the main diseases that affect the yield and quality of Brassica oleracea.In this study,306 breeding lines and cultivars from cauliflower,cabbage,broccoli,Chinese kale and kohlrabi were identified for clubroot resistance to race 4 of P.brassicae at seedling stage.The results showed that B.oleracea lacked resistant sources to clubroot,only one high resistant(HR)source was found in broccoli,the proportion of susceptible(S)and high susceptible(HS)materials were over 65%,and the proportion of medium resistant(MR)materials in both loose-curd cauliflower and cabbage were over 30%.According to the joint evaluation of resistance proportion and mean disease index,the overall resistance level of cabbage was higher than that of other varieties,and Chinese kale and kohlrabi showed the least resistance.In addition,hybrids derived from‘Xiangan 336’(R)×cabbage/broccoli and‘Tony’(MR)×broccoli were used to identify their clubroot resistance.The results indicated that all of hybrids showed reduced disease index compared to the male parents with relatively enhanced resistance and the lower disease index of the male parent,the higher proportion of higher resistance in the offspring.In view of this,the male and female parents both should be more highly resistant during the selection and mating of disease-resistant combinations in the future.This study will provide sources support and technical reference for the cultivation of resistant varieties to clubroot of B.oleracea.
作者
张小丽
单晓政
顾宏辉
文正华
赵振卿
姚星伟
盛小光
牛国保
王建升
孙德岭
ZHANG Xiaoli;SHAN Xiaozheng;GU Honghui;WEN Zhenghua;ZHAO Zhenqing;YAO Xingwei;SHENG Xiaoguang;NIU Guobao;WANG Jiansheng;SUN Deling(Tianjin Kernel Vegetable Research Institute, State Key Laboratory of Vegetable Germplasm Innovation, Tianjin Enterprise Key Laboratory of Vegetable Genetics & Breeding, Tianjin 300384, China;Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
出处
《植物保护》
CAS
CSCD
北大核心
2020年第5期193-199,210,共8页
Plant Protection
基金
国家重点研发计划(2017YFD0101805)
天津市青年科研人员创新研究与实验(2018014)
国家现代农业产业技术体系建设专项(CARS-23-A-07)。
关键词
甘蓝
根肿病
抗性
苗期
鉴定
Brassica oleracea
clubroot
resistance
seedling stage
identification
